High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe

Ching Yu Chou, Minglee Chu, Chi Fon Chang, Tsunai Yu, Tai huang Huang, Dimitris Sakellariou

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called “field-cycler”, which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed shuttling. In this communication, we demonstrate the first use of a sample shuttling device on a commercial high field standard bore NMR spectrometer, equipped with a commercial triple resonance cryogenically cooled NMR probe. The performance and robustness of the hardware operating in 1D and 2D field cycling experiments, as well as the impact of the sample shuttling time on the signal intensity are discussed.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalJournal of Biomolecular NMR
Volume66
Issue number3
DOIs
Publication statusPublished - 2016 Nov 1

Keywords

  • Cryo-probes
  • Field cycling
  • High-resolution
  • Protein dynamics
  • Relaxometry
  • Sensitivity

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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