Abstract
We report that gold/zinc oxide (Au/ZnO) nanocomposite films were effectively employed to enhance the performance of surface plasmon resonance (SPR) for the detection of tumor markers. Carbohydrate antigen 15.3 (CA15-3), a tumor marker for breast cancer, was chosen as a model analyte. We analyzed intensity response to the samples at various concentrations (0.0125 U/mL to 160 U/mL) in pleural fluid to evaluate the detection capability of the SPR biosensor based on Au/ZnO thin films. The linear range extended from 1 to 40 U/mL with a correlation coefficient of R<sup> 2</sup> = 0.991 and a limit of detection reaching 0.025 U/mL at a signal-to-noise ratio of 3:1. Compared with the degree of the shift in SPR intensity induced by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers was increased by at least 2 fold over that on the gold/chromium (Au/Cr) layers. In addition, we determined that the Au/ZnO layers allowed for a detection limit 4 times lower than the Au/Cr layers, which are in widespread use as the sensing interfaces in current SPR-based detectors. In conclusion, the use of Au/ ZnO films greatly enhanced the SPR signal yield for this bimolecular interaction and showed high sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 1207-1212 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 82 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2010 Feb 15 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
High-sensitivity detection of carbohydrate antigen 15-3 using a gold/zinc oxide thin film surface plasmon resonance-based biosensor. / Chang, Chia Chen; Chiu, Nan-Fu; Lin, David Shenhsiung; Chu-Su, Yu; Liang, Yang Hung; Lin, Chii Wann.
In: Analytical Chemistry, Vol. 82, No. 4, 15.02.2010, p. 1207-1212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-sensitivity detection of carbohydrate antigen 15-3 using a gold/zinc oxide thin film surface plasmon resonance-based biosensor
AU - Chang, Chia Chen
AU - Chiu, Nan-Fu
AU - Lin, David Shenhsiung
AU - Chu-Su, Yu
AU - Liang, Yang Hung
AU - Lin, Chii Wann
PY - 2010/2/15
Y1 - 2010/2/15
N2 - We report that gold/zinc oxide (Au/ZnO) nanocomposite films were effectively employed to enhance the performance of surface plasmon resonance (SPR) for the detection of tumor markers. Carbohydrate antigen 15.3 (CA15-3), a tumor marker for breast cancer, was chosen as a model analyte. We analyzed intensity response to the samples at various concentrations (0.0125 U/mL to 160 U/mL) in pleural fluid to evaluate the detection capability of the SPR biosensor based on Au/ZnO thin films. The linear range extended from 1 to 40 U/mL with a correlation coefficient of R 2 = 0.991 and a limit of detection reaching 0.025 U/mL at a signal-to-noise ratio of 3:1. Compared with the degree of the shift in SPR intensity induced by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers was increased by at least 2 fold over that on the gold/chromium (Au/Cr) layers. In addition, we determined that the Au/ZnO layers allowed for a detection limit 4 times lower than the Au/Cr layers, which are in widespread use as the sensing interfaces in current SPR-based detectors. In conclusion, the use of Au/ ZnO films greatly enhanced the SPR signal yield for this bimolecular interaction and showed high sensitivity.
AB - We report that gold/zinc oxide (Au/ZnO) nanocomposite films were effectively employed to enhance the performance of surface plasmon resonance (SPR) for the detection of tumor markers. Carbohydrate antigen 15.3 (CA15-3), a tumor marker for breast cancer, was chosen as a model analyte. We analyzed intensity response to the samples at various concentrations (0.0125 U/mL to 160 U/mL) in pleural fluid to evaluate the detection capability of the SPR biosensor based on Au/ZnO thin films. The linear range extended from 1 to 40 U/mL with a correlation coefficient of R 2 = 0.991 and a limit of detection reaching 0.025 U/mL at a signal-to-noise ratio of 3:1. Compared with the degree of the shift in SPR intensity induced by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers was increased by at least 2 fold over that on the gold/chromium (Au/Cr) layers. In addition, we determined that the Au/ZnO layers allowed for a detection limit 4 times lower than the Au/Cr layers, which are in widespread use as the sensing interfaces in current SPR-based detectors. In conclusion, the use of Au/ ZnO films greatly enhanced the SPR signal yield for this bimolecular interaction and showed high sensitivity.
UR - http://www.scopus.com/inward/record.url?scp=76849090412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76849090412&partnerID=8YFLogxK
U2 - 10.1021/ac901797j
DO - 10.1021/ac901797j
M3 - Article
C2 - 20102177
AN - SCOPUS:76849090412
VL - 82
SP - 1207
EP - 1212
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 4
ER -