TY - JOUR
T1 - Gsh-2, a murine homeobox gene expressed in the developing brain
AU - Hsieh-Li, Hsiu Mei
AU - Witte, David P.
AU - Szucsik, John C.
AU - Weinstein, Michael
AU - Li, Hung
AU - Potter, S. Steven
PY - 1995/4
Y1 - 1995/4
N2 - A novel murine dispersed homeobox gene, designated Gsh-2, is described. Analysis of cDNA sequence, including the full open reading frame, reveals an encoded homeodomain that is surprisingly similar to those of the Antennapedia-type clustered Hox genes. In addition, the encoded protein includes polyhistidine and polyalanine tracts, as observed for several other genes of developmental significance. In situ hybridizations showed Gsh-2 expression in the developing central nervous system, including the ganglionic eminences of the forebrain, the diencephalon, which gives rise to the thalamus and hypothalamus, and in the hindbrain. Furthermore, a random oligonucleotide selection and PCR amplification procedure was used to define a target DNA binding sequence, CNAATTAG, as a first step towards the identification of downstream target genes.
AB - A novel murine dispersed homeobox gene, designated Gsh-2, is described. Analysis of cDNA sequence, including the full open reading frame, reveals an encoded homeodomain that is surprisingly similar to those of the Antennapedia-type clustered Hox genes. In addition, the encoded protein includes polyhistidine and polyalanine tracts, as observed for several other genes of developmental significance. In situ hybridizations showed Gsh-2 expression in the developing central nervous system, including the ganglionic eminences of the forebrain, the diencephalon, which gives rise to the thalamus and hypothalamus, and in the hindbrain. Furthermore, a random oligonucleotide selection and PCR amplification procedure was used to define a target DNA binding sequence, CNAATTAG, as a first step towards the identification of downstream target genes.
KW - Brain development
KW - Homeobox gene
KW - Target sequence
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U2 - 10.1016/0925-4773(94)00334-J
DO - 10.1016/0925-4773(94)00334-J
M3 - Article
C2 - 7619729
AN - SCOPUS:0028912898
SN - 0925-4773
VL - 50
SP - 177
EP - 186
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 2-3
ER -