Gsh-2, a murine homeobox gene expressed in the developing brain

Hsiu Mei Hsieh, David P. Witte, John C. Szucsik, Michael Weinstein, Hung Li, S. Steven Potter

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

A novel murine dispersed homeobox gene, designated Gsh-2, is described. Analysis of cDNA sequence, including the full open reading frame, reveals an encoded homeodomain that is surprisingly similar to those of the Antennapedia-type clustered Hox genes. In addition, the encoded protein includes polyhistidine and polyalanine tracts, as observed for several other genes of developmental significance. In situ hybridizations showed Gsh-2 expression in the developing central nervous system, including the ganglionic eminences of the forebrain, the diencephalon, which gives rise to the thalamus and hypothalamus, and in the hindbrain. Furthermore, a random oligonucleotide selection and PCR amplification procedure was used to define a target DNA binding sequence, CNAATTAG, as a first step towards the identification of downstream target genes.

Original languageEnglish
Pages (from-to)177-186
Number of pages10
JournalMechanisms of Development
Volume50
Issue number2-3
DOIs
Publication statusPublished - 1995 Jan 1

Fingerprint

Homeobox Genes
Developmental Genes
Diencephalon
Rhombencephalon
Brain
Prosencephalon
Thalamus
Oligonucleotides
Open Reading Frames
Hypothalamus
In Situ Hybridization
Sequence Analysis
Central Nervous System
Complementary DNA
Polymerase Chain Reaction
Genes
Proteins
polyhistidine
polyalanine

Keywords

  • Brain development
  • Homeobox gene
  • Target sequence

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Hsieh, H. M., Witte, D. P., Szucsik, J. C., Weinstein, M., Li, H., & Potter, S. S. (1995). Gsh-2, a murine homeobox gene expressed in the developing brain. Mechanisms of Development, 50(2-3), 177-186. https://doi.org/10.1016/0925-4773(94)00334-J

Gsh-2, a murine homeobox gene expressed in the developing brain. / Hsieh, Hsiu Mei; Witte, David P.; Szucsik, John C.; Weinstein, Michael; Li, Hung; Potter, S. Steven.

In: Mechanisms of Development, Vol. 50, No. 2-3, 01.01.1995, p. 177-186.

Research output: Contribution to journalArticle

Hsieh, HM, Witte, DP, Szucsik, JC, Weinstein, M, Li, H & Potter, SS 1995, 'Gsh-2, a murine homeobox gene expressed in the developing brain', Mechanisms of Development, vol. 50, no. 2-3, pp. 177-186. https://doi.org/10.1016/0925-4773(94)00334-J
Hsieh, Hsiu Mei ; Witte, David P. ; Szucsik, John C. ; Weinstein, Michael ; Li, Hung ; Potter, S. Steven. / Gsh-2, a murine homeobox gene expressed in the developing brain. In: Mechanisms of Development. 1995 ; Vol. 50, No. 2-3. pp. 177-186.
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