Green fluorescent protein chromophore derivative suppresses ultraviolet A-induced JNK-signalling and apoptosis in keratinocytes and adverse effects in zebrafish embryos

Nan Lin Wu, Te An Lee, Sheng Fen Wang, Hsin Ju Li, Hui Ting Chen, Tun Cheng Chien, Chieh Chen Huang, Chi Feng Hung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Solar ultraviolet (UV) light has been recognized as the important environmental hazard and contributes to diverse skin damage such as cell death, photoageing and even carcinogenesis. Revelation of harmful responses attributed to UVA radiation has promoted the development of photoprotective agents against UVA-induced skin damage. In the present study, we tried to evaluate the potential protective effects of a synthetic green fluorescent protein (GFP) chromophore derivative, 4-chlorobenzyldene-1, 2-dimethylimidazolinone (Cl-BDI, called TC-22) on UVA- and UVB- induced stress responses in skin. The HaCaT keratinocytes were used to evaluate the cellular effects. Zebrafish (Danio rerio), which is regarded as a useful and cost-effective alternative to some mammalian models, was applied as the in vivo animal model. In HaCaT keratinocytes, TC-22 was able to obviously decrease UVA-induced cell death. Dissection of the UVA-induced signalling pathways revealed that TC-22 could suppress the activation of JNK and caspase 3, but not of ERK and p38. Reduction of UVA-induced cleavage of caspase 3 and sub-G1 phase accumulation by pretreatment of TC-22 was also observed. In zebrafish, we showed that UVA irradiation could decrease the survival and hatching rate, suppress heart beats of embryos and enhance the pigmentation of larvae. Pretreatment of TC-22 could significantly reverse UVA-induced the suppression in hatching of eggs and heart beating of embryos and also lowered the UVA-induced pigmentation in zebrafish. Collectively, we demonstrate that TC-22, a GFP chromophore derivative, can ameliorate the UVA-induced stress responses in both epidermal keratinocytes and zebrafish, suggesting the potential use of TC-22 in photoprotection in the future.

Original languageEnglish
Pages (from-to)983-990
Number of pages8
JournalExperimental Dermatology
Volume25
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Zebrafish
Chromophores
Green Fluorescent Proteins
Keratinocytes
Skin
Embryonic Structures
Cell death
Apoptosis
Derivatives
Caspase 3
Pigmentation
Dissection
Cell Death
Hazards
Animals
Chemical activation
Irradiation
G1 Phase
Radiation
Ultraviolet Rays

Keywords

  • GFP chromophore
  • keratinocyte
  • ultraviolet
  • zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Green fluorescent protein chromophore derivative suppresses ultraviolet A-induced JNK-signalling and apoptosis in keratinocytes and adverse effects in zebrafish embryos. / Wu, Nan Lin; Lee, Te An; Wang, Sheng Fen; Li, Hsin Ju; Chen, Hui Ting; Chien, Tun Cheng; Huang, Chieh Chen; Hung, Chi Feng.

In: Experimental Dermatology, Vol. 25, No. 12, 01.12.2016, p. 983-990.

Research output: Contribution to journalArticle

Wu, Nan Lin ; Lee, Te An ; Wang, Sheng Fen ; Li, Hsin Ju ; Chen, Hui Ting ; Chien, Tun Cheng ; Huang, Chieh Chen ; Hung, Chi Feng. / Green fluorescent protein chromophore derivative suppresses ultraviolet A-induced JNK-signalling and apoptosis in keratinocytes and adverse effects in zebrafish embryos. In: Experimental Dermatology. 2016 ; Vol. 25, No. 12. pp. 983-990.
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T1 - Green fluorescent protein chromophore derivative suppresses ultraviolet A-induced JNK-signalling and apoptosis in keratinocytes and adverse effects in zebrafish embryos

AU - Wu, Nan Lin

AU - Lee, Te An

AU - Wang, Sheng Fen

AU - Li, Hsin Ju

AU - Chen, Hui Ting

AU - Chien, Tun Cheng

AU - Huang, Chieh Chen

AU - Hung, Chi Feng

PY - 2016/12/1

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AB - Solar ultraviolet (UV) light has been recognized as the important environmental hazard and contributes to diverse skin damage such as cell death, photoageing and even carcinogenesis. Revelation of harmful responses attributed to UVA radiation has promoted the development of photoprotective agents against UVA-induced skin damage. In the present study, we tried to evaluate the potential protective effects of a synthetic green fluorescent protein (GFP) chromophore derivative, 4-chlorobenzyldene-1, 2-dimethylimidazolinone (Cl-BDI, called TC-22) on UVA- and UVB- induced stress responses in skin. The HaCaT keratinocytes were used to evaluate the cellular effects. Zebrafish (Danio rerio), which is regarded as a useful and cost-effective alternative to some mammalian models, was applied as the in vivo animal model. In HaCaT keratinocytes, TC-22 was able to obviously decrease UVA-induced cell death. Dissection of the UVA-induced signalling pathways revealed that TC-22 could suppress the activation of JNK and caspase 3, but not of ERK and p38. Reduction of UVA-induced cleavage of caspase 3 and sub-G1 phase accumulation by pretreatment of TC-22 was also observed. In zebrafish, we showed that UVA irradiation could decrease the survival and hatching rate, suppress heart beats of embryos and enhance the pigmentation of larvae. Pretreatment of TC-22 could significantly reverse UVA-induced the suppression in hatching of eggs and heart beating of embryos and also lowered the UVA-induced pigmentation in zebrafish. Collectively, we demonstrate that TC-22, a GFP chromophore derivative, can ameliorate the UVA-induced stress responses in both epidermal keratinocytes and zebrafish, suggesting the potential use of TC-22 in photoprotection in the future.

KW - GFP chromophore

KW - keratinocyte

KW - ultraviolet

KW - zebrafish

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