Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu; Po Len Liu; Kuang Jing Huang; Hui Min Wang; Kuo Feng Chang; Chon Kit Chou; Fang Rong Chang; Inn Wen Chong; Kang Fang; Jia Shing Chen; Hsueh Wei Chang; Yang Chang Wu

doi:10.1021/jf200566a

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu
, Po Len Liu
, Kuang Jing Huang
, Hui Min Wang
, Kuo Feng Chang
, Chon Kit Chou
, Fang Rong Chang
, Inn Wen Chong
, Kang Fang
, Jia Shing Chen
, Hsueh Wei Chang^*
, Yang Chang Wu

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

Original language	English
Pages (from-to)	4288-4293
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	59
Issue number	8
DOIs	https://doi.org/10.1021/jf200566a
Publication status	Published - 2011 Apr 27

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Goniothalamin
cell migration
comet assay
matrix metalloproteinase
non-small cell lung cancer

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf200566a

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Chiu, C. C., Liu, P. L., Huang, K. J., Wang, H. M., Chang, K. F., Chou, C. K., Chang, F. R., Chong, I. W., Fang, K., Chen, J. S., Chang, H. W., & Wu, Y. C. (2011). Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. Journal of Agricultural and Food Chemistry, 59(8), 4288-4293. https://doi.org/10.1021/jf200566a

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title = "Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability",

abstract = "We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.",

keywords = "Goniothalamin, cell migration, comet assay, matrix metalloproteinase, non-small cell lung cancer",

author = "Chiu, \{Chien Chih\} and Liu, \{Po Len\} and Huang, \{Kuang Jing\} and Wang, \{Hui Min\} and Chang, \{Kuo Feng\} and Chou, \{Chon Kit\} and Chang, \{Fang Rong\} and Chong, \{Inn Wen\} and Kang Fang and Chen, \{Jia Shing\} and Chang, \{Hsueh Wei\} and Wu, \{Yang Chang\}",

year = "2011",

month = apr,

day = "27",

doi = "10.1021/jf200566a",

language = "English",

volume = "59",

pages = "4288--4293",

journal = "Journal of Agricultural and Food Chemistry",

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T1 - Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

AU - Chiu, Chien Chih

AU - Liu, Po Len

AU - Huang, Kuang Jing

AU - Wang, Hui Min

AU - Chang, Kuo Feng

AU - Chou, Chon Kit

AU - Chang, Fang Rong

AU - Chong, Inn Wen

AU - Fang, Kang

AU - Chen, Jia Shing

AU - Chang, Hsueh Wei

AU - Wu, Yang Chang

PY - 2011/4/27

Y1 - 2011/4/27

N2 - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

AB - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

KW - Goniothalamin

KW - cell migration

KW - comet assay

KW - matrix metalloproteinase

KW - non-small cell lung cancer

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UR - https://www.scopus.com/pages/publications/79955033768#tab=citedBy

U2 - 10.1021/jf200566a

DO - 10.1021/jf200566a

M3 - Article

C2 - 21391609

AN - SCOPUS:79955033768

SN - 0021-8561

VL - 59

SP - 4288

EP - 4293

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 8

ER -

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Abstract

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Keywords

ASJC Scopus subject areas

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