Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu; Po Len Liu; Kuang Jing Huang; Hui Min Wang; Kuo Feng Chang; Chon Kit Chou; Fang Rong Chang; Inn Wen Chong; Kang Fang; Jia Shing Chen; Hsueh Wei Chang; Yang Chang Wu

doi:10.1021/jf200566a

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu, Po Len Liu, Kuang Jing Huang, Hui Min Wang, Kuo Feng Chang, Chon Kit Chou, Fang Rong Chang, Inn Wen Chong, Kang Fang, Jia Shing Chen, Hsueh Wei Chang^*, Yang Chang Wu

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

Original language	English
Pages (from-to)	4288-4293
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	59
Issue number	8
DOIs	https://doi.org/10.1021/jf200566a
Publication status	Published - 2011 Apr 27

Keywords

Goniothalamin
cell migration
comet assay
matrix metalloproteinase
non-small cell lung cancer

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/jf200566a

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Chiu, C. C., Liu, P. L., Huang, K. J., Wang, H. M., Chang, K. F., Chou, C. K., Chang, F. R., Chong, I. W., Fang, K., Chen, J. S., Chang, H. W., & Wu, Y. C. (2011). Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. Journal of Agricultural and Food Chemistry, 59(8), 4288-4293. https://doi.org/10.1021/jf200566a

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abstract = "We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.",

keywords = "Goniothalamin, cell migration, comet assay, matrix metalloproteinase, non-small cell lung cancer",

author = "Chiu, {Chien Chih} and Liu, {Po Len} and Huang, {Kuang Jing} and Wang, {Hui Min} and Chang, {Kuo Feng} and Chou, {Chon Kit} and Chang, {Fang Rong} and Chong, {Inn Wen} and Kang Fang and Chen, {Jia Shing} and Chang, {Hsueh Wei} and Wu, {Yang Chang}",

year = "2011",

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doi = "10.1021/jf200566a",

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T1 - Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

AU - Chiu, Chien Chih

AU - Liu, Po Len

AU - Huang, Kuang Jing

AU - Wang, Hui Min

AU - Chang, Kuo Feng

AU - Chou, Chon Kit

AU - Chang, Fang Rong

AU - Chong, Inn Wen

AU - Fang, Kang

AU - Chen, Jia Shing

AU - Chang, Hsueh Wei

AU - Wu, Yang Chang

PY - 2011/4/27

Y1 - 2011/4/27

N2 - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

AB - We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

KW - Goniothalamin

KW - cell migration

KW - comet assay

KW - matrix metalloproteinase

KW - non-small cell lung cancer

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Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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