Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability

Chien Chih Chiu, Po Len Liu, Kuang Jing Huang, Hui Min Wang, Kuo Feng Chang, Chon Kit Chou, Fang Rong Chang, Inn Wen Chong, Kang Fang, Jia Shing Chen, Hsueh Wei Chang, Yang Chang Wu

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.

Original languageEnglish
Pages (from-to)4288-4293
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number8
DOIs
Publication statusPublished - 2011 Apr 27

Fingerprint

human growth
lung neoplasms
DNA damage
DNA Damage
Lung Neoplasms
apoptosis
Cells
Apoptosis
DNA
Growth
Matrix Metalloproteinases
gelatinase A
gelatinase B
Assays
metalloproteinases
assays
tissue repair
cell movement
growth retardation
cell proliferation

Keywords

  • Goniothalamin
  • cell migration
  • comet assay
  • matrix metalloproteinase
  • non-small cell lung cancer

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. / Chiu, Chien Chih; Liu, Po Len; Huang, Kuang Jing; Wang, Hui Min; Chang, Kuo Feng; Chou, Chon Kit; Chang, Fang Rong; Chong, Inn Wen; Fang, Kang; Chen, Jia Shing; Chang, Hsueh Wei; Wu, Yang Chang.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 8, 27.04.2011, p. 4288-4293.

Research output: Contribution to journalArticle

Chiu, CC, Liu, PL, Huang, KJ, Wang, HM, Chang, KF, Chou, CK, Chang, FR, Chong, IW, Fang, K, Chen, JS, Chang, HW & Wu, YC 2011, 'Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability', Journal of Agricultural and Food Chemistry, vol. 59, no. 8, pp. 4288-4293. https://doi.org/10.1021/jf200566a
Chiu, Chien Chih ; Liu, Po Len ; Huang, Kuang Jing ; Wang, Hui Min ; Chang, Kuo Feng ; Chou, Chon Kit ; Chang, Fang Rong ; Chong, Inn Wen ; Fang, Kang ; Chen, Jia Shing ; Chang, Hsueh Wei ; Wu, Yang Chang. / Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 8. pp. 4288-4293.
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