GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions

Yi Ta Shao, Yung Che Tseng, Chia Hao Chang, Hong Young Yan, Pung Pung Hwang, Bertil Borg

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In vertebrates, reproduction is regulated by the brain-pituitary-gonad (BPG) axis, where the gonadotropin-releasing hormone (GnRH) is one of the key components. However, very little is known about the possible role of GnRH in the environmental and feedback control of fish reproduction. To investigate this, full-length gnrh2 (chicken GnRH II) and gnrh3 (salmon GnRH) sequences of male three-spined sticklebacks ( Gasterosteus aculeatus), which are clustered with the taxa of the same GnRH type as other Euteleostei, were cloned and annotated. gnrh1 is absent in this species. The mRNA levels of gnrh2 and gnrh3 in the sticklebacks' brain were measured under breeding and post-breeding conditions as well as in castrated and sham-operated breeding fish and castrated/sham-operated fish kept under long-day (LD 16:8) and short-day (LD 8:16) conditions. Fully breeding males had considerably higher mRNA levels of gnrh2 and gnrh3 in the thalamus ( Th) and in the telencephalon and preoptic area ( T+POA), respectively, than post-breeding males. Sham-operated breeding males have higher gnrh3 mRNA levels than the corresponding castrated males. Moreover, higher gnrh2 mRNA levels in the Th and higher gnrh3 mRNA levels in the T+POA and hypothalamus ( HypTh) were also found in long-day sham-operated males than in sham-operated fish kept under an inhibitory short day photoperiod. Nevertheless, gnrh2 and gnrh3 mRNA levels were not up-regulated in castrated males kept under long-day photoperiod, which suggests that positive feedbacks on the brain-pituitary-gonad axis are necessary for this response.

Original languageEnglish
Pages (from-to)6-17
Number of pages12
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume180
DOIs
Publication statusPublished - 2015 Feb 1

Fingerprint

Smegmamorpha
Gonadotropin-Releasing Hormone
Breeding
Fish
Messenger RNA
Fishes
Brain
Photoperiod
Gonads
Thalamus
Reproduction
Telencephalon
Preoptic Area
Feedback control
Hypothalamus
Vertebrates
Chickens
Feedback

Keywords

  • Feedback
  • GnRH2
  • GnRH3
  • Gonadectomy
  • MRNA
  • Photoperiod
  • Stickleback

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions. / Shao, Yi Ta; Tseng, Yung Che; Chang, Chia Hao; Yan, Hong Young; Hwang, Pung Pung; Borg, Bertil.

In: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Vol. 180, 01.02.2015, p. 6-17.

Research output: Contribution to journalArticle

@article{7409d5bc28284b199cfc1a5e60cda919,
title = "GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions",
abstract = "In vertebrates, reproduction is regulated by the brain-pituitary-gonad (BPG) axis, where the gonadotropin-releasing hormone (GnRH) is one of the key components. However, very little is known about the possible role of GnRH in the environmental and feedback control of fish reproduction. To investigate this, full-length gnrh2 (chicken GnRH II) and gnrh3 (salmon GnRH) sequences of male three-spined sticklebacks ( Gasterosteus aculeatus), which are clustered with the taxa of the same GnRH type as other Euteleostei, were cloned and annotated. gnrh1 is absent in this species. The mRNA levels of gnrh2 and gnrh3 in the sticklebacks' brain were measured under breeding and post-breeding conditions as well as in castrated and sham-operated breeding fish and castrated/sham-operated fish kept under long-day (LD 16:8) and short-day (LD 8:16) conditions. Fully breeding males had considerably higher mRNA levels of gnrh2 and gnrh3 in the thalamus ( Th) and in the telencephalon and preoptic area ( T+POA), respectively, than post-breeding males. Sham-operated breeding males have higher gnrh3 mRNA levels than the corresponding castrated males. Moreover, higher gnrh2 mRNA levels in the Th and higher gnrh3 mRNA levels in the T+POA and hypothalamus ( HypTh) were also found in long-day sham-operated males than in sham-operated fish kept under an inhibitory short day photoperiod. Nevertheless, gnrh2 and gnrh3 mRNA levels were not up-regulated in castrated males kept under long-day photoperiod, which suggests that positive feedbacks on the brain-pituitary-gonad axis are necessary for this response.",
keywords = "Feedback, GnRH2, GnRH3, Gonadectomy, MRNA, Photoperiod, Stickleback",
author = "Shao, {Yi Ta} and Tseng, {Yung Che} and Chang, {Chia Hao} and Yan, {Hong Young} and Hwang, {Pung Pung} and Bertil Borg",
year = "2015",
month = "2",
day = "1",
doi = "10.1016/j.cbpa.2014.10.008",
language = "English",
volume = "180",
pages = "6--17",
journal = "Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology",
issn = "1095-6433",

}

TY - JOUR

T1 - GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions

AU - Shao, Yi Ta

AU - Tseng, Yung Che

AU - Chang, Chia Hao

AU - Yan, Hong Young

AU - Hwang, Pung Pung

AU - Borg, Bertil

PY - 2015/2/1

Y1 - 2015/2/1

N2 - In vertebrates, reproduction is regulated by the brain-pituitary-gonad (BPG) axis, where the gonadotropin-releasing hormone (GnRH) is one of the key components. However, very little is known about the possible role of GnRH in the environmental and feedback control of fish reproduction. To investigate this, full-length gnrh2 (chicken GnRH II) and gnrh3 (salmon GnRH) sequences of male three-spined sticklebacks ( Gasterosteus aculeatus), which are clustered with the taxa of the same GnRH type as other Euteleostei, were cloned and annotated. gnrh1 is absent in this species. The mRNA levels of gnrh2 and gnrh3 in the sticklebacks' brain were measured under breeding and post-breeding conditions as well as in castrated and sham-operated breeding fish and castrated/sham-operated fish kept under long-day (LD 16:8) and short-day (LD 8:16) conditions. Fully breeding males had considerably higher mRNA levels of gnrh2 and gnrh3 in the thalamus ( Th) and in the telencephalon and preoptic area ( T+POA), respectively, than post-breeding males. Sham-operated breeding males have higher gnrh3 mRNA levels than the corresponding castrated males. Moreover, higher gnrh2 mRNA levels in the Th and higher gnrh3 mRNA levels in the T+POA and hypothalamus ( HypTh) were also found in long-day sham-operated males than in sham-operated fish kept under an inhibitory short day photoperiod. Nevertheless, gnrh2 and gnrh3 mRNA levels were not up-regulated in castrated males kept under long-day photoperiod, which suggests that positive feedbacks on the brain-pituitary-gonad axis are necessary for this response.

AB - In vertebrates, reproduction is regulated by the brain-pituitary-gonad (BPG) axis, where the gonadotropin-releasing hormone (GnRH) is one of the key components. However, very little is known about the possible role of GnRH in the environmental and feedback control of fish reproduction. To investigate this, full-length gnrh2 (chicken GnRH II) and gnrh3 (salmon GnRH) sequences of male three-spined sticklebacks ( Gasterosteus aculeatus), which are clustered with the taxa of the same GnRH type as other Euteleostei, were cloned and annotated. gnrh1 is absent in this species. The mRNA levels of gnrh2 and gnrh3 in the sticklebacks' brain were measured under breeding and post-breeding conditions as well as in castrated and sham-operated breeding fish and castrated/sham-operated fish kept under long-day (LD 16:8) and short-day (LD 8:16) conditions. Fully breeding males had considerably higher mRNA levels of gnrh2 and gnrh3 in the thalamus ( Th) and in the telencephalon and preoptic area ( T+POA), respectively, than post-breeding males. Sham-operated breeding males have higher gnrh3 mRNA levels than the corresponding castrated males. Moreover, higher gnrh2 mRNA levels in the Th and higher gnrh3 mRNA levels in the T+POA and hypothalamus ( HypTh) were also found in long-day sham-operated males than in sham-operated fish kept under an inhibitory short day photoperiod. Nevertheless, gnrh2 and gnrh3 mRNA levels were not up-regulated in castrated males kept under long-day photoperiod, which suggests that positive feedbacks on the brain-pituitary-gonad axis are necessary for this response.

KW - Feedback

KW - GnRH2

KW - GnRH3

KW - Gonadectomy

KW - MRNA

KW - Photoperiod

KW - Stickleback

UR - http://www.scopus.com/inward/record.url?scp=84911945752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911945752&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2014.10.008

DO - 10.1016/j.cbpa.2014.10.008

M3 - Article

AN - SCOPUS:84911945752

VL - 180

SP - 6

EP - 17

JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

SN - 1095-6433

ER -