Globotriose-functionalized gold nanoparticles as multivalent probes for Shiga-like toxin

Yuh Yih Chien, Mi Dan Jan, Avijit Kumar Adak, Hsiao Chien Tzeng, Yen Ping Lin, Yu Ju Chen, Ken Tseng Wang, Chien Tien Chen, Chia Chun Chen, Chun Cheng Lin

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82 Citations (Scopus)

Abstract

Compared to monovalent carbohydrates, multivalent carbohydrate ligands exhibit significantly enhanced binding affinities to their interacting proteins. Here, we report globotriose (Pk ligand)-functionalized gold nanoparticle (AuNP) probes for the investigation of multivalent interactions with the B5 subunit of Shiga-like toxin I (B-Slt). Six Pk-ligand-encapsulated AuNPs (Pk-AuNPs) of varying particle size and linker length were synthesized and evaluated for their potential as multivalent affinity probes by using a surface plasmon resonance competition assay. The affinity of these probes for the interacting proteins was greatly affected by nanoparticle size, linker length, and ligand density on nanoparticle surface. For example, the 20-nm 20-Pk-l-AuNP, which had a relatively long linker showed a >108-fold increase in affinity compared with the mono Pk ligand. This intrinsic high-affinity AuNP probe specifically captured the recombinant B-Slt from Escherichia coli lysate, and the resulting purity of the B-Slt was > 95 %. We also developed a robust Pk-AuNP-based detection method for Slt-I by combining the technique with silver enhancement.

Original languageEnglish
Pages (from-to)1100-1109
Number of pages10
JournalChemBioChem
Volume9
Issue number7
DOIs
Publication statusPublished - 2008 May 5

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Keywords

  • Glycoconjugates
  • Multivalent interaction
  • P antigen
  • Shiga-like toxin
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Chien, Y. Y., Jan, M. D., Adak, A. K., Tzeng, H. C., Lin, Y. P., Chen, Y. J., Wang, K. T., Chen, C. T., Chen, C. C., & Lin, C. C. (2008). Globotriose-functionalized gold nanoparticles as multivalent probes for Shiga-like toxin. ChemBioChem, 9(7), 1100-1109. https://doi.org/10.1002/cbic.200700590