Geographical variation and differential selection modes of paralogs of chloroplast small heat shock protein genes in Machilus kusanoi (Lauraceae)

Jeng Der Chung, Tsan Piao Lin, Yu Pin Cheng, Shih-Ying Hwang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The hydrophobicity of amino acid residues in the α-crystallin domain (ACD) of small heat shock proteins (sHSPs) is thought to be important for polypeptide binding and play key roles in substrate protection. A molecular population approach was applied in an attempt to understand the geographic variations and selective forces exerted on the chloroplast sHSP gene duplicates (CPsHSP-1 and CPsHSP-2) of Machilus kusanoi. In total, 84 individuals from 17 sampling sites were used in this investigation. Five haplotypes, including synonymous and nonsynonymous substitutions, were found for CPsHSP-1. However, only one synonymous substitution was found for CPsHSP-2. The conservation of CPsHSP-2 might be related to the action of purifying selection. In contrast, the wide distribution of several CPsHSP-1 haplotypes in different populations and the significantly positive value of Tajima's D suggested that balancing selection has governed the evolution of CPsHSP-1. The functional novelty of the paralogs of CPsHSPs was inferred from the hydrophobicity profile analysis and functional divergence test. Changes in hydrophobicity profile are also observed for the allelic variants of CPsHSP-1, and the hydrophbicity shift might have played important roles in expanding substrate specificity of the CPsHSP-1 as molecular chaperones.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalBotanical Studies
Volume51
Issue number1
Publication statusPublished - 2010 Jan 1

Fingerprint

Lauraceae
hydrophobicity
geographical variation
heat shock proteins
chloroplasts
haplotypes
crystallins
duplicate genes
molecular chaperones
genes
substrate specificity
polypeptides
amino acids
Machilus
testing
sampling

Keywords

  • Chloroplast small heat shock protein
  • Gene duplication
  • Hydrophobicity shift
  • Machilus kusanoi
  • Paralogs

ASJC Scopus subject areas

  • Plant Science

Cite this

Geographical variation and differential selection modes of paralogs of chloroplast small heat shock protein genes in Machilus kusanoi (Lauraceae). / Chung, Jeng Der; Lin, Tsan Piao; Cheng, Yu Pin; Hwang, Shih-Ying.

In: Botanical Studies, Vol. 51, No. 1, 01.01.2010, p. 27-36.

Research output: Contribution to journalArticle

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