Functional conservation of zinc-finger homeodomain gene zfh1/SIP1 in Drosophila heart development

Margaret Liu, Mingtsan Su, Gary E. Lyons, Rolf Bodmer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Comparative genetic studies of diverse animal model systems have revealed that similar developmental mechanisms operate across the Metazoa. In many cases, the genes from one organism can functionally replace homologues in other phyla, a result consistent with a high degree of evolutionarily conserved gene function. We investigated functional conservation among the Drosophila zinc-finger homeodomain protein 1 (zfh1) and its mouse functional homologue Smad-interacting protein 1 (SIP1). Northern blot analyses of SIP1 expression patterns detected three novel variants (8.3, 2.7, and 1.9 kb) in addition to the previously described 5.3 kb SIP1 transcript. The two shorter novel SIP1 transcripts were encountered only in developing embryos and both lacked zinc-finger clusters or homeodomain regions. The SIP1 transcripts showed complex embryonic expression patterns consistent with that observed for Drosophila zfh1. They were highly expressed in the developing nervous systems and in a number of mesoderm-derived tissues including lungs, heart, developing myotomes, skeletal muscle, and visceral smooth muscle. The expression of the mammalian 5.3 kb SIP1 transcript in Drosophila zfh1 null mutant embryos completely restored normal heart development in the fly, demonstrating their functional equivalence in cardiogenic pathways. Our present data, together with the previously described heart defects associated with both SIP1 and Drosophila zfh1 mutations, solidify the conclusion that the zfh1 family members participate in an evolutionary conserved program of metazoan cardiogenesis.

Original languageEnglish
Pages (from-to)683-693
Number of pages11
JournalDevelopment Genes and Evolution
Volume216
Issue number11
DOIs
Publication statusPublished - 2006 Nov 1

Fingerprint

Smad Proteins
homeodomain proteins
Homeodomain Proteins
zinc finger motif
Zinc Fingers
Drosophila
zinc
heart
protein
gene
Genes
genes
proteins
embryo (animal)
Embryonic Structures
Animalia
Mesoderm
embryo
nervous system
Northern blotting

Keywords

  • Cardiac
  • Hirschsprung disease
  • Smad
  • ZFHX1B

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Functional conservation of zinc-finger homeodomain gene zfh1/SIP1 in Drosophila heart development. / Liu, Margaret; Su, Mingtsan; Lyons, Gary E.; Bodmer, Rolf.

In: Development Genes and Evolution, Vol. 216, No. 11, 01.11.2006, p. 683-693.

Research output: Contribution to journalArticle

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