Folding of the protein domain hbSBD

Maksim Kouza, Chi Fon Chang, Shura Hayryan, Tsan Hung Yu, Mai Suan Li, Tai Huang Huang, Chin Kun Hu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The folding of the α-helix domain hbSBD of the mammalian mitochondrial branched-chain α-ketoacid dehydrogenase complex is studied by the circular dichroism technique in absence of urea. Thermal denaturation is used to evaluate various thermodynamic parameters defining the equilibrium unfolding, which is well described by the two-state model with the folding temperature TF = 317.8 ± 1.95 K and the enthalpy change ΔHG = 19.67 ±2.67 kcal/mol. The folding is also studied numerically using the off-lattice coarse-grained Go model and the Langevin dynamics. The obtained results, including the population of the native basin, the free-energy landscape as a function of the number of native contacts, and the folding kinetics, also suggest that the hbSBD domain is a two-state folder. These results are consistent with the biological function of hbSBD in branched-chain α-ketoacid dehydrogenase.

Original languageEnglish
Pages (from-to)3353-3361
Number of pages9
JournalBiophysical Journal
Volume89
Issue number5
DOIs
Publication statusPublished - 2005 Nov

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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Circular Dichroism
Thermodynamics
Urea
Hot Temperature
Temperature
Population
Protein Domains

ASJC Scopus subject areas

  • Biophysics

Cite this

Kouza, M., Chang, C. F., Hayryan, S., Yu, T. H., Li, M. S., Huang, T. H., & Hu, C. K. (2005). Folding of the protein domain hbSBD. Biophysical Journal, 89(5), 3353-3361. https://doi.org/10.1529/biophysj.105.065151

Folding of the protein domain hbSBD. / Kouza, Maksim; Chang, Chi Fon; Hayryan, Shura; Yu, Tsan Hung; Li, Mai Suan; Huang, Tai Huang; Hu, Chin Kun.

In: Biophysical Journal, Vol. 89, No. 5, 11.2005, p. 3353-3361.

Research output: Contribution to journalArticle

Kouza, M, Chang, CF, Hayryan, S, Yu, TH, Li, MS, Huang, TH & Hu, CK 2005, 'Folding of the protein domain hbSBD', Biophysical Journal, vol. 89, no. 5, pp. 3353-3361. https://doi.org/10.1529/biophysj.105.065151
Kouza M, Chang CF, Hayryan S, Yu TH, Li MS, Huang TH et al. Folding of the protein domain hbSBD. Biophysical Journal. 2005 Nov;89(5):3353-3361. https://doi.org/10.1529/biophysj.105.065151
Kouza, Maksim ; Chang, Chi Fon ; Hayryan, Shura ; Yu, Tsan Hung ; Li, Mai Suan ; Huang, Tai Huang ; Hu, Chin Kun. / Folding of the protein domain hbSBD. In: Biophysical Journal. 2005 ; Vol. 89, No. 5. pp. 3353-3361.
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