Flavones isolated from scutellariae radix suppress propionibacterium acnes-induced cytokine production in vitro and in vivo

Po Jung Tsai, Wen Cheng Huang, Ming Chi Hsieh, Ping Jyun Sung, Yueh Hsiung Kuo, Wen Huey Wu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Scutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluating the effect of Scutellariae radix extract and purified components isolated from it on inflammation induced by P. acnes in vitro and in vivo. The results showed the ethyl acetate (EA) soluble fraction from the partition of crude ethanolic extract from Scutellariae radix inhibited P. acnes-induced interleukin IL-8 and IL-1 production in human monocytic THP-1 cells. Seven flavones were isolated from the EA fraction by repeated chromatographies, and identified as 5,7-dihydroxy-6-methoxyflavone (FL1, oroxylin), 5,7-dihydroxy-8-methoxyflavone (FL2, wogonin), 5-hydroxy-7,8-dimethoxyflavone (FL3, 7-O-methylwogonin), 5,61-dihydroxy-6,7,8,21-tetramethoxy flavone (FL4, skullcapflavone II), 5,7,41-trihydroxy-8-methoxyflavone (FL5), 5,21,61-trihydroxy-7,8-dimethoxyflavone (FL6, viscidulin II), and 5,7,21,51-tetrahydroxy-8,61-dimethoxyflavone (FL7, ganhuangenin). They all significantly suppressed P. acnes-induced IL-8 and IL-1β production in THP-1 cells, and FL2 exerted the strongest effect with half maximal inhibition (IC50) values of 8.7 and 4.9 μM, respectively. Concomitant intradermal injection of each of the seven flavones (20 μg) with P. acnes effectively attenuated P. acnes-induced ear swelling, and decreased the production of IL-6 and tumor necrosis factor-α in ear homogenates. Our results suggested that all the seven flavones can be potential therapeutic agents against P. acnes-induced skin inflammation.

Original languageEnglish
Article number15
JournalMolecules
Volume21
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Scutellaria baicalensis
Flavones
Propionibacterium acnes
flavone
ear
Cytokines
Interleukin-8
Interleukin-1
acetates
interleukins
necrosis
Interleukins
chromatography
Chromatography
cells
Complex Mixtures
swelling
Swelling
Inflammation
partitions

Keywords

  • Anti-inflammation
  • Chinese herb
  • Flavone
  • Propionibacterium acnes
  • Scutellariae radix

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Flavones isolated from scutellariae radix suppress propionibacterium acnes-induced cytokine production in vitro and in vivo. / Tsai, Po Jung; Huang, Wen Cheng; Hsieh, Ming Chi; Sung, Ping Jyun; Kuo, Yueh Hsiung; Wu, Wen Huey.

In: Molecules, Vol. 21, No. 1, 15, 01.01.2016.

Research output: Contribution to journalArticle

Tsai, Po Jung ; Huang, Wen Cheng ; Hsieh, Ming Chi ; Sung, Ping Jyun ; Kuo, Yueh Hsiung ; Wu, Wen Huey. / Flavones isolated from scutellariae radix suppress propionibacterium acnes-induced cytokine production in vitro and in vivo. In: Molecules. 2016 ; Vol. 21, No. 1.
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abstract = "Scutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluating the effect of Scutellariae radix extract and purified components isolated from it on inflammation induced by P. acnes in vitro and in vivo. The results showed the ethyl acetate (EA) soluble fraction from the partition of crude ethanolic extract from Scutellariae radix inhibited P. acnes-induced interleukin IL-8 and IL-1 production in human monocytic THP-1 cells. Seven flavones were isolated from the EA fraction by repeated chromatographies, and identified as 5,7-dihydroxy-6-methoxyflavone (FL1, oroxylin), 5,7-dihydroxy-8-methoxyflavone (FL2, wogonin), 5-hydroxy-7,8-dimethoxyflavone (FL3, 7-O-methylwogonin), 5,61-dihydroxy-6,7,8,21-tetramethoxy flavone (FL4, skullcapflavone II), 5,7,41-trihydroxy-8-methoxyflavone (FL5), 5,21,61-trihydroxy-7,8-dimethoxyflavone (FL6, viscidulin II), and 5,7,21,51-tetrahydroxy-8,61-dimethoxyflavone (FL7, ganhuangenin). They all significantly suppressed P. acnes-induced IL-8 and IL-1β production in THP-1 cells, and FL2 exerted the strongest effect with half maximal inhibition (IC50) values of 8.7 and 4.9 μM, respectively. Concomitant intradermal injection of each of the seven flavones (20 μg) with P. acnes effectively attenuated P. acnes-induced ear swelling, and decreased the production of IL-6 and tumor necrosis factor-α in ear homogenates. Our results suggested that all the seven flavones can be potential therapeutic agents against P. acnes-induced skin inflammation.",
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