FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster

Kuang Lung Hsueh, Liang Kun Yu, Yung Han Chen, Ya Hsin Cheng, Yin Cheng Hsieh, Shyue chu Ke, Kuo Wei Hung, Chun Jung Chen, Tai Huang Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Iron is essential for pathogen survival, virulence, and colonization. Feo is suggested to function as the ferrous iron (Fe2+) transporter. The enterobacterial Feo system is composed of 3 proteins: FeoB is the indispensable component and is a large membrane protein likely to function as a permease; FeoA is a small Src homology 3 (SH3) domain protein that interacts with FeoB; FeoC is a winged-helix protein containing 4 conserved Cys residues in a sequence suitable for harboring a putative iron-sulfur (Fe-S) cluster. The presence of an iron-sulfur cluster on FeoC has never been shown experimentally. We report that under anaerobic conditions, the recombinant Klebsiella pneumoniae FeoC (KpFeoC) exhibited hyperfine-shifted nuclear magnetic resonance (NMR) and a UV-visible (UV-Vis) absorbance spectrum characteristic of a paramagnetic center. The electron paramagnetic resonance (EPR) and extended X-ray absorption fine structure (EXAFS) results were consistent only with the [4Fe-4S] clusters. Substituting the cysteinyl sulfur with oxygen resulted in significantly reduced cluster stability, establishing the roles of these cysteines as the ligands for the Fe-S cluster. When exposed to oxygen, the [4Fe-4S] cluster degraded to [3Fe-4S] and eventually disappeared. We propose that KpFeoC may regulate the function of the Feo transporter through the oxygen- or iron-sensitive coordination of the Fe-S cluster.

Original languageEnglish
Pages (from-to)4726-4734
Number of pages9
JournalJournal of Bacteriology
Volume195
Issue number20
DOIs
Publication statusPublished - 2013 Oct 18

Fingerprint

Klebsiella pneumoniae
Iron
Sulfur
Oxygen
Proteins
src Homology Domains
Membrane Transport Proteins
Electron Spin Resonance Spectroscopy
Cysteine
Virulence
Membrane Proteins
Magnetic Resonance Spectroscopy
X-Rays
Ligands

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Hsueh, K. L., Yu, L. K., Chen, Y. H., Cheng, Y. H., Hsieh, Y. C., Ke, S. C., ... Huang, T. H. (2013). FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. Journal of Bacteriology, 195(20), 4726-4734. https://doi.org/10.1128/JB.00687-13

FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. / Hsueh, Kuang Lung; Yu, Liang Kun; Chen, Yung Han; Cheng, Ya Hsin; Hsieh, Yin Cheng; Ke, Shyue chu; Hung, Kuo Wei; Chen, Chun Jung; Huang, Tai Huang.

In: Journal of Bacteriology, Vol. 195, No. 20, 18.10.2013, p. 4726-4734.

Research output: Contribution to journalArticle

Hsueh, KL, Yu, LK, Chen, YH, Cheng, YH, Hsieh, YC, Ke, SC, Hung, KW, Chen, CJ & Huang, TH 2013, 'FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster', Journal of Bacteriology, vol. 195, no. 20, pp. 4726-4734. https://doi.org/10.1128/JB.00687-13
Hsueh KL, Yu LK, Chen YH, Cheng YH, Hsieh YC, Ke SC et al. FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. Journal of Bacteriology. 2013 Oct 18;195(20):4726-4734. https://doi.org/10.1128/JB.00687-13
Hsueh, Kuang Lung ; Yu, Liang Kun ; Chen, Yung Han ; Cheng, Ya Hsin ; Hsieh, Yin Cheng ; Ke, Shyue chu ; Hung, Kuo Wei ; Chen, Chun Jung ; Huang, Tai Huang. / FeoC from Klebsiella pneumoniae contains a [4Fe-4S] cluster. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 20. pp. 4726-4734.
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