Fast multipoint immobilized MOF bioreactor

Wan Ling Liu, Cheng You Wu, Chien Yu Chen, Brenda Singco, Chia Her Lin*, Hsi Ya Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

An enzyme-NBD@MOF bioreactor with exemplary proteolytic performance, even after successive reuse and storage, was produced through a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution could be directly monitored from the fluorescence emission of the bioreactor. The dye molecular dimension plays a role in its overall performance. United they stand: An enzyme-NBD@MOF bioreactor (see figure) with exemplary proteolytic performance was produced by a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution can be directly monitored from the fluorescence emission of the bioreactor.

Original languageEnglish
Pages (from-to)8923-8928
Number of pages6
JournalChemistry - A European Journal
Volume20
Issue number29
DOIs
Publication statusPublished - 2014 Jul 14
Externally publishedYes

Keywords

  • biocatalysis
  • bioreactors
  • enzymes
  • immobilization
  • metal-organic frameworks

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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