Fast multipoint immobilization of lipase through chirall-proline on a MOF as a chiral bioreactor

Stephen Lirio, Yung Han Shih, Pamela Berilyn So, Li Hao Liu, Yun Ting Yen, Shuhei Furukawa*, Wan Ling Liu, Hsi Ya Huang, Chia Her Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In this paper, we describe the facile preparation of a chiral catalyst by the combination of the amino acid,l-proline (Pro), and the enzyme, porcine pancreas lipase (PPL), immobilized on a microporous metal-organic framework (PPL-Pro@MOF). The multipoint immobilization of PPL onto the MOF is made possible with the aid of Pro, which also provided a chiral environment for enhanced enantioselectivity. The application of the microporous MOF is pivotal in maintaining the catalytic activity of PPL, wherein it prevented the leaching of Pro during the catalytic reaction, leading to the enhanced activity of PPL. The prepared biocatalyst was applied in asymmetric carbon-carbon bond formation, demonstrating the potential of this simple approach for chemical transformations.

Original languageEnglish
Pages (from-to)1866-1873
Number of pages8
JournalDalton Transactions
Volume50
Issue number5
DOIs
Publication statusPublished - 2021 Feb 7

ASJC Scopus subject areas

  • Inorganic Chemistry

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