Expression of heat shock proteins (Hsps) in caprine bone marrow-derived mesenchymal stem cells

D. Jena; S. D. Kharche; K. Gururaj; S. P. Singh; Sonam Rani; A. Pachoori

doi:10.5958/0973-9718.2020.00010.0

Expression of heat shock proteins (Hsps) in caprine bone marrow-derived mesenchymal stem cells

D. Jena, S. D. Kharche, K. Gururaj, S. P. Singh, Sonam Rani, A. Pachoori

Research output: Contribution to journal › Article › peer-review

Abstract

A study was made to observe the relative expression of four heat shock proteins i.e. HSP₂₇, HSP₇₂, HSP₇₃ and HSP₉₀ on caprine bone marrow derived mesenchymal stem cells (MSCs). Bone marrow was collected from 1 to 2 year old healthy goats and cultured up to passage 3, after which the cells were used for RNA isolation and cDNA preparation to study real time expression analysis. The result showed that HSP₂₇, HSP₇₂ and HSP₉₀ expressed significantly higher (P<0.05) as compared to HSP₇₃. HSP₇₃-an important constituent of apoptosis, surprisingly down-regulated whereas HSP₇₂ was significantly up-regulated. In conclusion, the expression of certain HSPs as mentioned above on the caprine bone marrow derived MSCs confirms the stemness and anti-apoptosis nature of the MSCs.

Original language	English
Pages (from-to)	128-131
Number of pages	4
Journal	Indian Journal of Small Ruminants
Volume	20
Issue number	1
DOIs	https://doi.org/10.5958/0973-9718.2020.00010.0
Publication status	Published - 2020 Apr
Externally published	Yes

Keywords

Bone marrow
Caprine
Heat shock proteins
Mesenchymal stem cells

ASJC Scopus subject areas

Animal Science and Zoology
Small Animals

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10.5958/0973-9718.2020.00010.0

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title = "Expression of heat shock proteins (Hsps) in caprine bone marrow-derived mesenchymal stem cells",

abstract = "A study was made to observe the relative expression of four heat shock proteins i.e. HSP27, HSP72, HSP73 and HSP90 on caprine bone marrow derived mesenchymal stem cells (MSCs). Bone marrow was collected from 1 to 2 year old healthy goats and cultured up to passage 3, after which the cells were used for RNA isolation and cDNA preparation to study real time expression analysis. The result showed that HSP27, HSP72 and HSP90 expressed significantly higher (P<0.05) as compared to HSP73. HSP73-an important constituent of apoptosis, surprisingly down-regulated whereas HSP72 was significantly up-regulated. In conclusion, the expression of certain HSPs as mentioned above on the caprine bone marrow derived MSCs confirms the stemness and anti-apoptosis nature of the MSCs.",

keywords = "Bone marrow, Caprine, Heat shock proteins, Mesenchymal stem cells",

author = "D. Jena and Kharche, {S. D.} and K. Gururaj and Singh, {S. P.} and Sonam Rani and A. Pachoori",

note = "Funding Information: The authors are thankful to the Assistant Director General, National Agricultural Research Fund, New Delhi and the Director, ICAR-Central Institute for Research on Goats, Makhdoom (Uttar Pradesh) for providing necessary facilities for the research work. Publisher Copyright: {\textcopyright} 2020, Indian Society for Sheep and Goat Production and Utilization. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = apr,

doi = "10.5958/0973-9718.2020.00010.0",

language = "English",

volume = "20",

pages = "128--131",

journal = "Indian Journal of Small Ruminants",

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publisher = "Indian Society for Sheep and Goat Production and Utilization",

number = "1",

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TY - JOUR

T1 - Expression of heat shock proteins (Hsps) in caprine bone marrow-derived mesenchymal stem cells

AU - Jena, D.

AU - Kharche, S. D.

AU - Gururaj, K.

AU - Singh, S. P.

AU - Rani, Sonam

AU - Pachoori, A.

N1 - Funding Information: The authors are thankful to the Assistant Director General, National Agricultural Research Fund, New Delhi and the Director, ICAR-Central Institute for Research on Goats, Makhdoom (Uttar Pradesh) for providing necessary facilities for the research work. Publisher Copyright: © 2020, Indian Society for Sheep and Goat Production and Utilization. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4

Y1 - 2020/4

N2 - A study was made to observe the relative expression of four heat shock proteins i.e. HSP27, HSP72, HSP73 and HSP90 on caprine bone marrow derived mesenchymal stem cells (MSCs). Bone marrow was collected from 1 to 2 year old healthy goats and cultured up to passage 3, after which the cells were used for RNA isolation and cDNA preparation to study real time expression analysis. The result showed that HSP27, HSP72 and HSP90 expressed significantly higher (P<0.05) as compared to HSP73. HSP73-an important constituent of apoptosis, surprisingly down-regulated whereas HSP72 was significantly up-regulated. In conclusion, the expression of certain HSPs as mentioned above on the caprine bone marrow derived MSCs confirms the stemness and anti-apoptosis nature of the MSCs.

AB - A study was made to observe the relative expression of four heat shock proteins i.e. HSP27, HSP72, HSP73 and HSP90 on caprine bone marrow derived mesenchymal stem cells (MSCs). Bone marrow was collected from 1 to 2 year old healthy goats and cultured up to passage 3, after which the cells were used for RNA isolation and cDNA preparation to study real time expression analysis. The result showed that HSP27, HSP72 and HSP90 expressed significantly higher (P<0.05) as compared to HSP73. HSP73-an important constituent of apoptosis, surprisingly down-regulated whereas HSP72 was significantly up-regulated. In conclusion, the expression of certain HSPs as mentioned above on the caprine bone marrow derived MSCs confirms the stemness and anti-apoptosis nature of the MSCs.

KW - Bone marrow

KW - Caprine

KW - Heat shock proteins

KW - Mesenchymal stem cells

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EP - 131

JO - Indian Journal of Small Ruminants

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IS - 1

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Expression of heat shock proteins (Hsps) in caprine bone marrow-derived mesenchymal stem cells

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Keywords

ASJC Scopus subject areas

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