TY - JOUR
T1 - Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay
AU - Huang, K. W.
AU - Yang, Shieh-Yueh
AU - Yu, C. Y.
AU - Chieh, J. J.
AU - Yang, Che Chuan
AU - Horng, Herng-Er
AU - Hong, Chin Yih
AU - Yang, Hong-Chang
AU - Wu, Chau Chung
PY - 2011/8
Y1 - 2011/8
N2 - To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
AB - To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
KW - Animal model
KW - Hepatocellular carcinoma
KW - Immunomagnetic reduction
KW - Magnetic nanoparticle
KW - Vascular endothelial growth factor
UR - http://www.scopus.com/inward/record.url?scp=80052886167&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052886167&partnerID=8YFLogxK
U2 - 10.1166/jbn.2011.1321
DO - 10.1166/jbn.2011.1321
M3 - Article
C2 - 21870457
AN - SCOPUS:80052886167
SN - 1550-7033
VL - 7
SP - 535
EP - 541
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
IS - 4
ER -
