Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

K. W. Huang; S. Y. Yang; C. Y. Yu; J. J. Chieh; Che Chuan Yang; Herng Er Horng; Chin Yih Hong; Hong Chang Yang; Chau Chung Wu

doi:10.1166/jbn.2011.1321

Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

K. W. Huang, S. Y. Yang, C. Y. Yu, J. J. Chieh, Che Chuan Yang, Herng Er Horng^*, Chin Yih Hong, Hong Chang Yang, Chau Chung Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

Original language	English
Pages (from-to)	535-541
Number of pages	7
Journal	Journal of Biomedical Nanotechnology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1166/jbn.2011.1321
Publication status	Published - 2011 Aug

Keywords

Animal model
Hepatocellular carcinoma
Immunomagnetic reduction
Magnetic nanoparticle
Vascular endothelial growth factor

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science
Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1166/jbn.2011.1321

Cite this

Huang, K. W., Yang, S. Y., Yu, C. Y., Chieh, J. J., Yang, C. C., Horng, H. E., Hong, C. Y., Yang, H. C., & Wu, C. C. (2011). Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay. Journal of Biomedical Nanotechnology, 7(4), 535-541. https://doi.org/10.1166/jbn.2011.1321

Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay. / Huang, K. W.; Yang, S. Y.; Yu, C. Y. et al.
In: Journal of Biomedical Nanotechnology, Vol. 7, No. 4, 08.2011, p. 535-541.

Research output: Contribution to journal › Article › peer-review

Huang, KW, Yang, SY, Yu, CY, Chieh, JJ, Yang, CC, Horng, HE, Hong, CY, Yang, HC & Wu, CC 2011, 'Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay', Journal of Biomedical Nanotechnology, vol. 7, no. 4, pp. 535-541. https://doi.org/10.1166/jbn.2011.1321

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abstract = "To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.",

keywords = "Animal model, Hepatocellular carcinoma, Immunomagnetic reduction, Magnetic nanoparticle, Vascular endothelial growth factor",

author = "Huang, {K. W.} and Yang, {S. Y.} and Yu, {C. Y.} and Chieh, {J. J.} and Yang, {Che Chuan} and Horng, {Herng Er} and Hong, {Chin Yih} and Yang, {Hong Chang} and Wu, {Chau Chung}",

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doi = "10.1166/jbn.2011.1321",

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AU - Yu, C. Y.

AU - Chieh, J. J.

AU - Yang, Che Chuan

AU - Horng, Herng Er

AU - Hong, Chin Yih

AU - Yang, Hong Chang

AU - Wu, Chau Chung

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N2 - To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

AB - To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.

KW - Animal model

KW - Hepatocellular carcinoma

KW - Immunomagnetic reduction

KW - Magnetic nanoparticle

KW - Vascular endothelial growth factor

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Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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