Experimental study on low-detection limit for immunomagnetic reduction assays by manipulating reagent entities

S. Y. Yang, C. C. Yang, H. E. Horng, B. Y. Shih, J. J. Chieh, C. Y. Hong, H. C. Yang

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Abstract

The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are ${\rm Fe}-{3}{\rm O} 4 magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

Original languageEnglish
Article number6451290
Pages (from-to)65-68
Number of pages4
JournalIEEE Transactions on Nanobioscience
Volume12
Issue number2
DOIs
Publication statusPublished - 2013 Jun 12

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Keywords

  • Immunomagnetic reduction
  • magnetic nanoparticles
  • reagent

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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