Experimental study on low-detection limit for immunomagnetic reduction assays by manipulating reagent entities

S. Y. Yang, C. C. Yang, H. E. Horng, B. Y. Shih, J. J. Chieh, C. Y. Hong, H. C. Yang

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are ${\rm Fe}-{3}{\rm O} 4 magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

    Original languageEnglish
    Article number6451290
    Pages (from-to)65-68
    Number of pages4
    JournalIEEE Transactions on Nanobioscience
    Volume12
    Issue number2
    DOIs
    Publication statusPublished - 2013 Jun 12

    Keywords

    • Immunomagnetic reduction
    • magnetic nanoparticles
    • reagent

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Medicine (miscellaneous)
    • Biomedical Engineering
    • Pharmaceutical Science
    • Computer Science Applications
    • Electrical and Electronic Engineering

    Fingerprint Dive into the research topics of 'Experimental study on low-detection limit for immunomagnetic reduction assays by manipulating reagent entities'. Together they form a unique fingerprint.

    Cite this