Evaluation of anti-invasion effect of resveratrol and related methoxy analogues on human hepatocarcinoma cells

Chia Jui Weng, Cheng Feng Wu, Hsiao Wen Huang, Chi Hao Wu, Chi Tang Ho, Gow Chin Yen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)


Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is also highly metastatic. Metastasis is considered to be the major cause of death in cancer patients. Resveratrol (3,5,4'-trihydroxystilbene) and related analogues have been reported as candidates to prevent cancer growth and invasion. The bioactivity of resveratrol-related analogues could be altered due to the presence and positioning of methoxy groups on the basic resveratrol chemical structure. This study investigated the effects and mechanism of action of resveratrol and its methoxy analogues on invasion of human hepatocarcinoma cells. The migratory and invasive abilities of phorbol 12-myristate 13-acetate (PMA)-treated HepG2 and PMA-untreated Hep3B cells were both reduced in a dose-dependent manner by treatment with resveratrol and 3,5,4'-trimethoxy-frans- stilbene (MR-3). Upon incubation of PMA-treated HepG2 cells with resveratrol (0-50 μM) or MR-3 (0-50 μM), the MMP-9 activity decreased but TIMP-1 protein increased in a dose-dependent manner. With resveratrol (0-50 μM) or MR-3 (0-1 μM) treatment on PMA-untreated Hep3B cells, both of the MMP-9 and MMP-2 activities decreased but TIMP-2 protein increased in a dose-dependent manner. These results suggest that resveratrol and its related methoxy analogue MR-3 might exert anti-invasive activity against hepatoma cells through regulation of MMP-2, MMP-9, TIMP-1, and TIMP-2. Further analysis with semiquantitative RT-PCR showed that the regulation of MMP-9 and TIMP-2 expressions by resveratrol and MR-3 in hepatoma cells may be on the transcriptional level but on the translational or posttranslational level for TIMP-1.

Original languageEnglish
Pages (from-to)2886-2894
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Issue number5
Publication statusPublished - 2010 Mar 10
Externally publishedYes


  • Invasion
  • MMPs
  • MR-3
  • Resveratrol
  • TIMPs
  • uPA

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


Dive into the research topics of 'Evaluation of anti-invasion effect of resveratrol and related methoxy analogues on human hepatocarcinoma cells'. Together they form a unique fingerprint.

Cite this