Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish

Tien Chien Pan, Bo Kai Liao, Chang Jen Huang, Li Yih Lin, Pung Pung Hwang

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The purpose of the present work was to study the possible role of the epithelial Ca2+ channel (ECaC) in the Ca2+ uptake mechanism in developing zebrafish (Danio rerio). With rapid amplification of cDNA ends, full-length cDNA encoding the ECaC of zebrafish (zECaC) was cloned and sequenced. The cloned zECaC was 2,578 bp in length and encoded a protein of 709 amino acids that showed up to 73% identity with previously described vertebrate ECaCs. The zECaC was found to be expressed in all tissues examined and began to be expressed in the skin covering the yolk sac of embryos at 24 h postfertilization (hpf). zECaC-expressing cells expanded to cover the skin of the entire yolk sac after embryonic development and began to occur in the gill filaments at 96 hpf, and thereafter zECaC-expressing cells rapidly increased in both gills and yolk sac skin. Corresponding to ECaC expression profile, the Ca2+ influx and content began to increase at 36-72 hpf. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater caused upregulation of the whole body Ca2+ influx and zECaC expression in both gills and skin. Colocalization of zECaC mRNA and the Na+-K +-ATPase α-subunit (a marker for mitochondria-rich cells) indicated that only a portion of the mitochondria-rich cells expressed zECaC mRNA. These results suggest that the zECaC plays a key role in Ca2+ absorption in developing zebrafish.

Original languageEnglish
Pages (from-to)R1202-R1211
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume289
Issue number4 58-4
DOIs
Publication statusPublished - 2005 Oct 1

Fingerprint

Zebrafish
Yolk Sac
Skin
Mitochondria
Embryonic Structures
Complementary DNA
Messenger RNA
Fresh Water
Embryonic Development
Vertebrates
Up-Regulation
Amino Acids
Proteins

Keywords

  • Calcium influx
  • Low calcium, mitochondria-rich cells
  • Zebrafish embryo

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish. / Pan, Tien Chien; Liao, Bo Kai; Huang, Chang Jen; Lin, Li Yih; Hwang, Pung Pung.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 289, No. 4 58-4, 01.10.2005, p. R1202-R1211.

Research output: Contribution to journalArticle

Pan, Tien Chien ; Liao, Bo Kai ; Huang, Chang Jen ; Lin, Li Yih ; Hwang, Pung Pung. / Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2005 ; Vol. 289, No. 4 58-4. pp. R1202-R1211.
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AB - The purpose of the present work was to study the possible role of the epithelial Ca2+ channel (ECaC) in the Ca2+ uptake mechanism in developing zebrafish (Danio rerio). With rapid amplification of cDNA ends, full-length cDNA encoding the ECaC of zebrafish (zECaC) was cloned and sequenced. The cloned zECaC was 2,578 bp in length and encoded a protein of 709 amino acids that showed up to 73% identity with previously described vertebrate ECaCs. The zECaC was found to be expressed in all tissues examined and began to be expressed in the skin covering the yolk sac of embryos at 24 h postfertilization (hpf). zECaC-expressing cells expanded to cover the skin of the entire yolk sac after embryonic development and began to occur in the gill filaments at 96 hpf, and thereafter zECaC-expressing cells rapidly increased in both gills and yolk sac skin. Corresponding to ECaC expression profile, the Ca2+ influx and content began to increase at 36-72 hpf. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater caused upregulation of the whole body Ca2+ influx and zECaC expression in both gills and skin. Colocalization of zECaC mRNA and the Na+-K +-ATPase α-subunit (a marker for mitochondria-rich cells) indicated that only a portion of the mitochondria-rich cells expressed zECaC mRNA. These results suggest that the zECaC plays a key role in Ca2+ absorption in developing zebrafish.

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