Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

Nan Fu Chiu, Chi Chu Chen, Cheng Du Yang, Yu Sheng Kao, Wei Ren Wu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.

Original languageEnglish
Article number152
JournalNanoscale Research Letters
Volume13
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

immunoassay
Graphite
bioinstrumentation
Biosensors
Gold
Oxides
Graphene
Labels
graphene
Biomarkers
gold
Nanoparticles
nanoparticles
oxides
biomarkers
Nanocomposites
Proteins
nanocomposites
Nanosheets
Surface plasmon resonance

Keywords

  • Gold nanoparticle (AuNP)
  • Graphene oxide sheet (GO)
  • Immunoassays
  • Localized surface plasmon resonance (LSPR)

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay. / Chiu, Nan Fu; Chen, Chi Chu; Yang, Cheng Du; Kao, Yu Sheng; Wu, Wei Ren.

In: Nanoscale Research Letters, Vol. 13, 152, 01.01.2018.

Research output: Contribution to journalArticle

@article{883a2390d2824e379d9c9bfdd5965ffa,
title = "Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay",
abstract = "In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.",
keywords = "Gold nanoparticle (AuNP), Graphene oxide sheet (GO), Immunoassays, Localized surface plasmon resonance (LSPR)",
author = "Chiu, {Nan Fu} and Chen, {Chi Chu} and Yang, {Cheng Du} and Kao, {Yu Sheng} and Wu, {Wei Ren}",
year = "2018",
month = "1",
day = "1",
doi = "10.1186/s11671-018-2565-7",
language = "English",
volume = "13",
journal = "Nanoscale Research Letters",
issn = "1931-7573",
publisher = "Springer New York",

}

TY - JOUR

T1 - Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

AU - Chiu, Nan Fu

AU - Chen, Chi Chu

AU - Yang, Cheng Du

AU - Kao, Yu Sheng

AU - Wu, Wei Ren

PY - 2018/1/1

Y1 - 2018/1/1

N2 - In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.

AB - In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.

KW - Gold nanoparticle (AuNP)

KW - Graphene oxide sheet (GO)

KW - Immunoassays

KW - Localized surface plasmon resonance (LSPR)

UR - http://www.scopus.com/inward/record.url?scp=85047191158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047191158&partnerID=8YFLogxK

U2 - 10.1186/s11671-018-2565-7

DO - 10.1186/s11671-018-2565-7

M3 - Article

VL - 13

JO - Nanoscale Research Letters

JF - Nanoscale Research Letters

SN - 1931-7573

M1 - 152

ER -