Endothelin-1 regulates hh +-atpase-dependent transepithelial hh + secretion in zebrafish

Ying Jey Guh, Yung Che Tseng, Chao Yew Yang, Pung Pung Hwang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Endothelin-1 (EDN1) is an important regulator of H + secretion in the mammalian kidney. EDN1 enhances renal tubule H +-ATPase activity, but the underlying mechanism remains unclear. To further elucidate the role of EDN1 in vertebrates' acid-base regulation, the present study used zebrafish as the model to examine the effects of EDN1 and its receptors on transepithelial H + secretion. Expression of EDN1 and one of its receptors, EDNRAa, was stimulated in zebrafish acclimated to acidic water. A noninvasive scanning ion-selective electrode technique was used toshowthat edn1 overexpression enhancesH +secretion in embryonic skin at 3 days post fertilization.EDNRAa loss of function significantly decreased EDN1- and acid-induced H + secretion. Abrogationof EDN1-enhanced H + secretion by a vacuolar H +-ATPase inhibitor (bafilomycin A1) suggests thatEDN1 exerts its action by regulating the H +-ATPase- mediated H + secretion. EDN1 does not appearto affect H + secretion through either altering the abundance of H +-ATPase or affecting the celldifferentiation of H +-ATPase-rich ionocytes, because the reduction in secretion upon ednraaknockdown was not accompanied by decreased expression of H +-ATPase or reduced H +- ATPaserichcell density. These findings provide evidence that EDN1 signaling is involved in acid-baseregulation in zebrafish and enhance our understanding of EDN1 regulation of transepithelial H +secretion in vertebrates.

Original languageEnglish
Pages (from-to)1728-1737
Number of pages10
JournalEndocrinology
Volume155
Issue number5
DOIs
Publication statusPublished - 2014 May

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Endothelin-1
Zebrafish
Adenosine Triphosphatases
Proton-Translocating ATPases
Vertebrates
Vacuolar Proton-Translocating ATPases
Ion-Selective Electrodes
Kidney
Endothelin A Receptors
Acids
Fertilization
Skin
Water

ASJC Scopus subject areas

  • Endocrinology

Cite this

Endothelin-1 regulates hh +-atpase-dependent transepithelial hh + secretion in zebrafish. / Guh, Ying Jey; Tseng, Yung Che; Yang, Chao Yew; Hwang, Pung Pung.

In: Endocrinology, Vol. 155, No. 5, 05.2014, p. 1728-1737.

Research output: Contribution to journalArticle

Guh, Ying Jey ; Tseng, Yung Che ; Yang, Chao Yew ; Hwang, Pung Pung. / Endothelin-1 regulates hh +-atpase-dependent transepithelial hh + secretion in zebrafish. In: Endocrinology. 2014 ; Vol. 155, No. 5. pp. 1728-1737.
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