Emblica officinalis gaertn. attentuates N-nitrosodiethylamine-induced apoptosis, autophagy, and inflammation in rat livers

Kuo Hsin Chen, Bor Ru Lin, Chiang-Ting Chien, Chien Hsin Ho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Inflammation and oxidative stress contribute to liver injury. Amla (Emblica officinalis Gaertn.) is rich in vitamin C, gallic acid, flavonoids, and tannins, which may protect against hepatoxicity-induced liver injury. We elucidated the effects of supplementary Amla (100 mg/kg of body weight) on N-nitrosodiethylamine-induced injury by evaluating reactive oxygen species (ROS) responses in the liver and bile, the degree of accumulated leukocytes and Kupffer cell infiltration, 3-nitrotyrosine and 4-hydroxynonenal stains, apoptosis and autophagy, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transpeptidase (γ-GT) levels, and antioxidant/oxidant enzymes in rats. Amla was more potent than vitamin C in scavenging O 2 -•, hydrogen peroxide, and nitric oxide. N-Nitrosodiethylamine increased ROS production in liver and bile, hepatic Kupffer cell and leukocyte infiltration, 3-nitrotyrosine and 4-hydroxynonenal accumulations, apoptosis and autophagy, and plasma ALT, AST, and γ-GT levels in the rats, decreased hepatic manganese superoxide dismutase (MnSOD) and catalase protein expressions, and enhanced inducible nitric oxide synthase (iNOS) and cytochrome P450 2E1 (CYP2E1) protein expressions. Amla significantly preserved MnSOD and catalase expressions and decreased iNOS and CYP2E1 protein expressions in N-nitrosodiethylamine-treated livers. Amla decreased N-nitrosodiethylamine-enhanced hepatic apoptosis and autophagy appearances via down-regulation of the Bax/Bcl-2 ratio and Beclin-1 expression. Thus Amla supplementation counteracts N-nitrosodiethylamine-induced liver injury via its antioxidant, anti-inflammation, anti-apoptosis, and anti-autophagy properties.

Original languageEnglish
Pages (from-to)746-755
Number of pages10
JournalJournal of Medicinal Food
Volume14
Issue number7-8
DOIs
Publication statusPublished - 2011 Jul 1

Fingerprint

Phyllanthus emblica
Diethylnitrosamine
Autophagy
Apoptosis
Inflammation
Liver
Cytochrome P-450 CYP2E1
Kupffer Cells
Wounds and Injuries
Nitric Oxide Synthase Type II
Aspartate Aminotransferases
Alanine Transaminase
Bile
Catalase
Ascorbic Acid
Superoxide Dismutase
Reactive Oxygen Species
Leukocytes
Antioxidants
Gallic Acid

Keywords

  • antioxidant
  • apoptosis
  • autophagy
  • inflammation
  • liver injury
  • reactive oxygen species

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Emblica officinalis gaertn. attentuates N-nitrosodiethylamine-induced apoptosis, autophagy, and inflammation in rat livers. / Chen, Kuo Hsin; Lin, Bor Ru; Chien, Chiang-Ting; Ho, Chien Hsin.

In: Journal of Medicinal Food, Vol. 14, No. 7-8, 01.07.2011, p. 746-755.

Research output: Contribution to journalArticle

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