Elevated p53 and p21waf1 mRNA expression in blood lymphocytes from lung cancer patients with chemoresistance

Chuen Ming Shih, Kwunmin Chen, Yu Chieh Wang, Pei Jung Lee, Yi Ching Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Clinical and experimental studies suggest that alteration of the expression level of the p53 gene and other damage responsive genes may be associated with chemoresistance in cancer patients. Methods: The present study evaluated the differences of the basal levels of lymphocytic p53 and p21waf1 mRNA expression collected before receiving cisplatin-based chemotherapy between 48 chemo-ineffective lung cancer patients and 39 chemo-effective lung cancer patients using an optimized semi-quantitative multiplex reverse transcriptase-polymerase chain reaction (RT-PCR). Results: The data indicated that the mean mRNA level of p53 gene in chemo-ineffective patients (0.66) was 26.9% higher than that of the chemo-effective patients (0.52) with statistical significance (P = 0.03), and a significantly higher level of p21waf1 mRNA expression in the chemo-ineffective patients (P = 0.03) was also observed. In addition, by the multiplex long quantitative PCR analysis, we demonstrated that chemo-ineffective and chemo-effective patients have similar amounts of UV damage on their p53 gene of lymphocyte DNA through equal UV treatment. Conclusion: Our results suggest that elevated levels of p53/p21waf1 mRNA in blood lymphocytes collected before chemotherapy may predict the chemoresponses of lung cancer patients.

Original languageEnglish
Pages (from-to)366-370
Number of pages5
JournalCancer Detection and Prevention
Volume31
Issue number5
DOIs
Publication statusPublished - 2007 Dec 4

Keywords

  • Chemoresistance
  • Lung cancer
  • Lymphocyte
  • p21waf1 gene
  • p53 gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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