Electron tunneling in proteins: Coupling through a β strand

Ralf Langen, I-Jy Chang, Juris P. Germanas, John H. Richards, Jay R. Winkler, Harry B. Gray

Research output: Contribution to journalArticle

318 Citations (Scopus)

Abstract

Electron coupling through a β strand has been investigated by measurement of the intramolecular electron-transfer (ET) rates in ruthenium-modified derivatives of the β barrel blue copper protein Pseudomonas aeruginosa azurin. Surface histidines, introduced on the methionine-121 β strand by mutagenesis, were modified with a Ru(2,2′-bipyridine)2(imidazole)2+ complex. The Cu+ to Ru3+ rate constants yielded a distance-decay constant of 1.1 per angstrom, a value close to the distance-decay constant of 1.0 per angstrom predicted for electron tunneling through an idealized β strand. Activation-less ET rate constants in combination with a tunneling-pathway analysis of the structures of azurin and cytochrome c confirm that there is a generally efficient network for coupling the internal (native) redox center to the surface of both proteins.

Original languageEnglish
Pages (from-to)1733-1735
Number of pages3
JournalScience
Volume268
Issue number5218
DOIs
Publication statusPublished - 1995 Jan 1

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Azurin
Electrons
Proteins
Ruthenium
Cytochromes c
Histidine
Mutagenesis
Methionine
Pseudomonas aeruginosa
Oxidation-Reduction
Copper
Membrane Proteins

ASJC Scopus subject areas

  • General

Cite this

Langen, R., Chang, I-J., Germanas, J. P., Richards, J. H., Winkler, J. R., & Gray, H. B. (1995). Electron tunneling in proteins: Coupling through a β strand. Science, 268(5218), 1733-1735. https://doi.org/10.1126/science.7792598

Electron tunneling in proteins : Coupling through a β strand. / Langen, Ralf; Chang, I-Jy; Germanas, Juris P.; Richards, John H.; Winkler, Jay R.; Gray, Harry B.

In: Science, Vol. 268, No. 5218, 01.01.1995, p. 1733-1735.

Research output: Contribution to journalArticle

Langen, R, Chang, I-J, Germanas, JP, Richards, JH, Winkler, JR & Gray, HB 1995, 'Electron tunneling in proteins: Coupling through a β strand', Science, vol. 268, no. 5218, pp. 1733-1735. https://doi.org/10.1126/science.7792598
Langen R, Chang I-J, Germanas JP, Richards JH, Winkler JR, Gray HB. Electron tunneling in proteins: Coupling through a β strand. Science. 1995 Jan 1;268(5218):1733-1735. https://doi.org/10.1126/science.7792598
Langen, Ralf ; Chang, I-Jy ; Germanas, Juris P. ; Richards, John H. ; Winkler, Jay R. ; Gray, Harry B. / Electron tunneling in proteins : Coupling through a β strand. In: Science. 1995 ; Vol. 268, No. 5218. pp. 1733-1735.
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