Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis

Kuo Chin Huang, Shih Ping Hsu, Chih Ching Yang, Pu Ou-Yang, Kun Tai Lee, Shinkatsu Morisawa, Kazumichi Otsubo, Chiang Ting Chien

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background. T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4 + T lymphocytes. Antioxidant electrolysed-reduced water (ERW) was used as the dialysate in ESRD patients undergoing chronic HD to test for improved oxidative stress-related T-cell apoptosis, alterations of surface markers and intracellular cytokine profile.Methods. We evaluated apoptosis formation by annexin V, CD25-related surface markers, and cytokine ratio of Th1/Th2 in CD4 + T lymphocytes and Tc1/Tc2 in CD8 + T lymphocytes of 42 ESRD patients haemodialysed with ERW for 1 year.Results. In comparison to 12 healthy individuals, the ESRD patients had more T-cell apoptosis and less CD3 + , CD4 + and CD8 + T cells and CD25/CD69/CD94/CD3 + phenotypes at baseline. Lower intracellular IL-2 and IFN-γ levels in the Th1/CD4 + and Tc1/CD8 + cells and higher intracellular IL-4, IL-6 and IL-10 levels in the Th2/CD4 + and Tc2/CD8 + cells were also noted in the ESRD patients. After a 1-year ERW treatment, the patients had a decrease in T-cell apoptosis and increases in CD3 + , CD4 + and CD8 + cell numbers and CD25/CD69/CD94/CD3 + phenotypes in the T cells. The intracellular IL-2 and IFN-γ levels in the Th1/Tc1 cells significantly (P < 0.05) increased and the intracellular IL-4, IL-6 and IL-10 levels in the Th2/Tc2 cells decreased. Furthermore, the Th1/Th2 and Tc1/Tc2 cytokine ratios were improved toward a normal status.Conclusion. One-year ERW treatment effectively ameliorated T-cell apoptosis, altered CD25-related surface markers and intracellular cytokine profile in the HD patients. Â

Original languageEnglish
Pages (from-to)2730-2737
Number of pages8
JournalNephrology Dialysis Transplantation
Volume25
Issue number8
DOIs
Publication statusPublished - 2010 Aug

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Dialysis Solutions
Chronic Kidney Failure
Renal Dialysis
T-Lymphocytes
Water
Apoptosis
Cytokines
Water Purification
Interleukin-4
Interleukin-10
Interleukin-2
Interleukin-6
Oxidative Stress
Phenotype
Th2 Cells
Th1 Cells
Annexin A5
Cell Count
Antioxidants

Keywords

  • T-lymphocyte
  • apoptosis
  • electrolysed reduced water
  • end-stage renal disease
  • oxidative stress

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis. / Huang, Kuo Chin; Hsu, Shih Ping; Yang, Chih Ching; Ou-Yang, Pu; Lee, Kun Tai; Morisawa, Shinkatsu; Otsubo, Kazumichi; Chien, Chiang Ting.

In: Nephrology Dialysis Transplantation, Vol. 25, No. 8, 08.2010, p. 2730-2737.

Research output: Contribution to journalArticle

Huang, Kuo Chin ; Hsu, Shih Ping ; Yang, Chih Ching ; Ou-Yang, Pu ; Lee, Kun Tai ; Morisawa, Shinkatsu ; Otsubo, Kazumichi ; Chien, Chiang Ting. / Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis. In: Nephrology Dialysis Transplantation. 2010 ; Vol. 25, No. 8. pp. 2730-2737.
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abstract = "Background. T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4 + T lymphocytes. Antioxidant electrolysed-reduced water (ERW) was used as the dialysate in ESRD patients undergoing chronic HD to test for improved oxidative stress-related T-cell apoptosis, alterations of surface markers and intracellular cytokine profile.Methods. We evaluated apoptosis formation by annexin V, CD25-related surface markers, and cytokine ratio of Th1/Th2 in CD4 + T lymphocytes and Tc1/Tc2 in CD8 + T lymphocytes of 42 ESRD patients haemodialysed with ERW for 1 year.Results. In comparison to 12 healthy individuals, the ESRD patients had more T-cell apoptosis and less CD3 + , CD4 + and CD8 + T cells and CD25/CD69/CD94/CD3 + phenotypes at baseline. Lower intracellular IL-2 and IFN-{\^I}³ levels in the Th1/CD4 + and Tc1/CD8 + cells and higher intracellular IL-4, IL-6 and IL-10 levels in the Th2/CD4 + and Tc2/CD8 + cells were also noted in the ESRD patients. After a 1-year ERW treatment, the patients had a decrease in T-cell apoptosis and increases in CD3 + , CD4 + and CD8 + cell numbers and CD25/CD69/CD94/CD3 + phenotypes in the T cells. The intracellular IL-2 and IFN-{\^I}³ levels in the Th1/Tc1 cells significantly (P < 0.05) increased and the intracellular IL-4, IL-6 and IL-10 levels in the Th2/Tc2 cells decreased. Furthermore, the Th1/Th2 and Tc1/Tc2 cytokine ratios were improved toward a normal status.Conclusion. One-year ERW treatment effectively ameliorated T-cell apoptosis, altered CD25-related surface markers and intracellular cytokine profile in the HD patients. {\^A}",
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AU - Ou-Yang, Pu

AU - Lee, Kun Tai

AU - Morisawa, Shinkatsu

AU - Otsubo, Kazumichi

AU - Chien, Chiang Ting

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N2 - Background. T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4 + T lymphocytes. Antioxidant electrolysed-reduced water (ERW) was used as the dialysate in ESRD patients undergoing chronic HD to test for improved oxidative stress-related T-cell apoptosis, alterations of surface markers and intracellular cytokine profile.Methods. We evaluated apoptosis formation by annexin V, CD25-related surface markers, and cytokine ratio of Th1/Th2 in CD4 + T lymphocytes and Tc1/Tc2 in CD8 + T lymphocytes of 42 ESRD patients haemodialysed with ERW for 1 year.Results. In comparison to 12 healthy individuals, the ESRD patients had more T-cell apoptosis and less CD3 + , CD4 + and CD8 + T cells and CD25/CD69/CD94/CD3 + phenotypes at baseline. Lower intracellular IL-2 and IFN-γ levels in the Th1/CD4 + and Tc1/CD8 + cells and higher intracellular IL-4, IL-6 and IL-10 levels in the Th2/CD4 + and Tc2/CD8 + cells were also noted in the ESRD patients. After a 1-year ERW treatment, the patients had a decrease in T-cell apoptosis and increases in CD3 + , CD4 + and CD8 + cell numbers and CD25/CD69/CD94/CD3 + phenotypes in the T cells. The intracellular IL-2 and IFN-γ levels in the Th1/Tc1 cells significantly (P < 0.05) increased and the intracellular IL-4, IL-6 and IL-10 levels in the Th2/Tc2 cells decreased. Furthermore, the Th1/Th2 and Tc1/Tc2 cytokine ratios were improved toward a normal status.Conclusion. One-year ERW treatment effectively ameliorated T-cell apoptosis, altered CD25-related surface markers and intracellular cytokine profile in the HD patients. Â

AB - Background. T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4 + T lymphocytes. Antioxidant electrolysed-reduced water (ERW) was used as the dialysate in ESRD patients undergoing chronic HD to test for improved oxidative stress-related T-cell apoptosis, alterations of surface markers and intracellular cytokine profile.Methods. We evaluated apoptosis formation by annexin V, CD25-related surface markers, and cytokine ratio of Th1/Th2 in CD4 + T lymphocytes and Tc1/Tc2 in CD8 + T lymphocytes of 42 ESRD patients haemodialysed with ERW for 1 year.Results. In comparison to 12 healthy individuals, the ESRD patients had more T-cell apoptosis and less CD3 + , CD4 + and CD8 + T cells and CD25/CD69/CD94/CD3 + phenotypes at baseline. Lower intracellular IL-2 and IFN-γ levels in the Th1/CD4 + and Tc1/CD8 + cells and higher intracellular IL-4, IL-6 and IL-10 levels in the Th2/CD4 + and Tc2/CD8 + cells were also noted in the ESRD patients. After a 1-year ERW treatment, the patients had a decrease in T-cell apoptosis and increases in CD3 + , CD4 + and CD8 + cell numbers and CD25/CD69/CD94/CD3 + phenotypes in the T cells. The intracellular IL-2 and IFN-γ levels in the Th1/Tc1 cells significantly (P < 0.05) increased and the intracellular IL-4, IL-6 and IL-10 levels in the Th2/Tc2 cells decreased. Furthermore, the Th1/Th2 and Tc1/Tc2 cytokine ratios were improved toward a normal status.Conclusion. One-year ERW treatment effectively ameliorated T-cell apoptosis, altered CD25-related surface markers and intracellular cytokine profile in the HD patients. Â

KW - T-lymphocyte

KW - apoptosis

KW - electrolysed reduced water

KW - end-stage renal disease

KW - oxidative stress

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