Electrical detection of protein using gold nanoparticles and nanogap electrodes

Chien Ying Tsai, Tien Li Chang, Ramesh Uppala, Chun Chi Chen, K. O. Fu-Hsiang, Ping Hei Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


A method of electrically detecting of protein described is developed using self-assembled multilayer gold nanoparticles (AuNPs) on a SiO2/Si substrate between gold electrodes. Electrical measurements are performed at room temperature using a probe station. A monoclonal antibody is immobilized on the top surface of the first layer of AuNPs (14nm). The second layer of AuNPs is formed through specific binding among a target antigen [hepatitis C virus, (HCV)], the monoclonal antibody, and the conjugate of a AuNP-polyclonal antibody. Once the specific binding among the monoclonal antibody, target antigen, and polyclonal antibody occurs, a significant electric current is detected through multilayer self-assembled gold nanoparticles between nanogap electrodes. No significant current (<1 pA) can be measured through a monolayer of AuNPs. A significant difference between the IV curves of the monolayer and the multilayer of AuNPs is used to identify whether the target antigen exists in the tested sample.

Original languageEnglish
Pages (from-to)5711-5716
Number of pages6
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Issue number7 B
Publication statusPublished - 2005 Jul 26
Externally publishedYes


  • Electrical detection
  • Gold nanoparticle
  • Multilayer
  • Protein chip
  • Self-assembled monolayer

ASJC Scopus subject areas

  • General Engineering
  • General Physics and Astronomy


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