Electrical detection of DNA using gold and magnetic nanoparticles and bio bar-code DNA between nanogap electrodes

Tien Li Chang, Chien Ying Tsai, Chih Chen Sun, Ramesh Uppala, Chun Chi Chen, Chun Hung Lin, Ping Hei Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This paper presents an electrical detection method on a DNA biochip that employs a novel approach for ultra sensitive detection of DNA using self-assembled gold nanoparticles and bio-bar-code-based amplification (BCA) DNA. The experimental study relies on three-components oligonucleotide-modified gold nanoparticles, single-component oligonucleotide-modified magnetic nanoparticles and subsequent detection of amplified target DNA in the form of bio-bar-code ssDNA (single strand DNA) using a chip-based detection method. In this study, the BCA technique measures the bar-code DNA rather than the target DNA. There withal, the DNA chips with nanogap electrodes are fabricated by electron-beam lithography. The gap distance and an electrode height are 300 and 65 nm, respectively. Here, the surface between the electrodes and multilayer of gold nanoparticles is established by the hybridization among single strand BCA, the second capture DNA (C2DNA) and the second probe DNA (P2DNA). Measurable current through nanogap electrodes can be obtained over multilayer gold nanoparticles. In this way, magnetic nanoparticles and bio-bar-code DNA are used to amplify obtainable current through nanogap electrodes from the extremely low concentration of target DNA. The detective concentration of target DNA with electrical DNA biosensor is as low as 1 fM for the analysis of current-voltage curves.

Original languageEnglish
Pages (from-to)1630-1633
Number of pages4
JournalMicroelectronic Engineering
Volume83
Issue number4-9 SPEC. ISS.
DOIs
Publication statusPublished - 2006 Apr 1
Externally publishedYes

Keywords

  • Bio-bar-code DNA detection
  • DNA Biochip
  • Electrical Detection
  • Gold nanoparticles
  • Magnetic nanoparticles
  • Self-assembly

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering

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