TY - JOUR
T1 - Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo
AU - Tseng, Deng Yu
AU - Chou, Ming Yi
AU - Tseng, Yung Che
AU - Hsiao, Chung Der
AU - Huang, Chang Jen
AU - Kaneko, Toyoji
AU - Hwang, Pung Pung
PY - 2009/3
Y1 - 2009/3
N2 - Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca2+ content, Ca 2+ influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca2+ uptake in zebrafish embryos.
AB - Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca2+ content, Ca 2+ influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca2+ uptake in zebrafish embryos.
KW - Ca influx
KW - Ca uptake
UR - http://www.scopus.com/inward/record.url?scp=64149129834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64149129834&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.90742.2008
DO - 10.1152/ajpregu.90742.2008
M3 - Article
C2 - 19073903
AN - SCOPUS:64149129834
SN - 0363-6119
VL - 296
SP - R549-R557
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 3
ER -