Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo

Deng Yu Tseng, Ming Yi Chou, Yung Che Tseng, Chung Der Hsiao, Chang Jen Huang, Toyoji Kaneko, Pung Pung Hwang

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca2+ content, Ca 2+ influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca2+ uptake in zebrafish embryos.

Original languageEnglish
Pages (from-to)R549-R557
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume296
Issue number3
DOIs
Publication statusPublished - 2009 Mar 1

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Zebrafish
Embryonic Structures
Calcium
Morpholinos
Messenger RNA
Microinjections
Fresh Water
Disulfides
teleocalcin
Glycoproteins
Fishes
Down-Regulation
Kidney
Gene Expression
Muscles
Skin
Brain
Proteins

Keywords

  • Ca influx
  • Ca uptake

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. / Tseng, Deng Yu; Chou, Ming Yi; Tseng, Yung Che; Hsiao, Chung Der; Huang, Chang Jen; Kaneko, Toyoji; Hwang, Pung Pung.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 296, No. 3, 01.03.2009, p. R549-R557.

Research output: Contribution to journalArticle

Tseng, Deng Yu ; Chou, Ming Yi ; Tseng, Yung Che ; Hsiao, Chung Der ; Huang, Chang Jen ; Kaneko, Toyoji ; Hwang, Pung Pung. / Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2009 ; Vol. 296, No. 3. pp. R549-R557.
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