Effective prevention and treatment of Helicobacter pylori infection using a combination of catechins and sialic acid in AGS cells and BALB/c mice

Jyh Chin Yang, Chia Tung Shun, Chiang Ting Chien, Teh Hong Wang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The increasing emergence of Helicobacter pylori strains resistant to antibiotics may cause unsuccessful treatment. An alternative agent or mixture with anti-H. pylori effect is urgently required to reduce H. pylori infection. We explored the preventive and therapeutic potential of a combination of catechins and sialic acid on H. pylori-infected human gastric cells in vitro and in mice in vivo. We evaluated the anti-H. pylori activity of catechins and/or sialic acid using the agar dilution and checkerboard methods. The effect of catechins and/or sialic acid on H. pylori infection-induced oxidative stress and apoptosis/autophagy in cell culture was explored using an ultrasensitive chemiluminescence analyzer, immunocytochemistry, and Western blotting. Specific pathogen-free BALB/c mice were divided into uninfected control, infected control, pretreated, and post-treated groups. The effects of catechins/sialic acid were determined by histology and immunocytochemistry. The combination of catechins and sialic acid showed synergistic or additive anti-H. pylori activity and significantly reduced inducible nitric oxide synthase expression and Bax/Bcl-2-mediated apoptosis but enhanced Beclin-1-mediated autophagy. All mice infected with H. pylori displayed gastritis and accumulation of 3-nitrotyrosine and 4-hydroxynonenal. Pretreatment with catechins/sialic acid completely prevented H. pylori infection and resulted in normal histology. Post-treatment with catechins/sialic acid decreased the bacterial load and gastritis score and eradicated up to 60% of H. pylori infections in a dose-dependent manner. This is the first demonstration to our knowledge of a nonprobiotic, nonantibiotic treatment that is 100% effective in preventing and has promising possibilities for treating H. pylori infection. Further studies are needed to confirm this result in humans.

Original languageEnglish
Pages (from-to)2084-2090
Number of pages7
JournalJournal of Nutrition
Volume138
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

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Catechin
Helicobacter Infections
N-Acetylneuraminic Acid
Helicobacter pylori
Therapeutics
Autophagy
Gastritis
Histology
Immunohistochemistry
Apoptosis
Specific Pathogen-Free Organisms
Bacterial Load
Nitric Oxide Synthase Type II
Luminescence
Agar
Stomach
Oxidative Stress
Cell Culture Techniques
Western Blotting
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Effective prevention and treatment of Helicobacter pylori infection using a combination of catechins and sialic acid in AGS cells and BALB/c mice. / Yang, Jyh Chin; Shun, Chia Tung; Chien, Chiang Ting; Wang, Teh Hong.

In: Journal of Nutrition, Vol. 138, No. 11, 01.11.2008, p. 2084-2090.

Research output: Contribution to journalArticle

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