Effect of Times to Blood Processing on the Stability of Blood Proteins Associated with Dementia

Jui Feng Chang, Huei Chun Liu, H. H. Chen, Wen Ping Chen, Jian Lin Juang, Pei Ning Wang, Shieh Yueh Yang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background: The stability of proteins in the collecting tubes after blood draw is critical to the measured concentrations of the proteins. Although the guidelines issued by the Clinical and Laboratory Standards Institute (CLSI) suggest centrifugation should take place within 2 h of drawing blood, it is very difficult to follow these guidelines in hospitals or clinics. It is necessary to study the effect of times to blood processing on the stability of the proteins of interest. Methods: In this work, the plasma proteins of interest were those relevant to dementia, such as amyloid β 1-40 (Aβ1-40), Aβ1-42, Tau protein (Tau), and α-synuclein. The times to blood processing after blood draw ranged from 0.5 to 8 h. The storage temperatures of blood were room temperature (approx. 25°C) and 30°C. After storage, blood samples were centrifuged at room temperature to obtain plasma samples. Ultrasensitive immunomagnetic reduction was applied to assay these proteins in the plasma. Results: The levels of plasma Aβ1-40, Tau, and α-synuclein did not significantly change until 8 h after blood draw when stored at room temperature. Plasma Aβ1-42 levels did not change significantly after 8 h of storage at room temperature before blood processing. Higher storage temperatures, such as 30°C, for blood samples accelerated the significant variations in the measured concentrations of Aβ1-40, Tau, and α-synuclein in plasma. Conclusion: According to these results, for clinical practice, it is suggested that blood samples be stored at room temperature for no longer than 4.5 h after blood draw until centrifugation for the assay of dementia biomarkers in plasma.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalDementia and Geriatric Cognitive Disorders
Volume49
Issue number3
DOIs
Publication statusPublished - 2020 Nov
Externally publishedYes

Keywords

  • Blood
  • Centrifugation
  • Dementia
  • Immunomagnetic reduction
  • Stability

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Cognitive Neuroscience
  • Psychiatry and Mental health

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