Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI

Jason Chia Hsien Cheng, Ang Yuan, Jyh Horng Chen, Yi Chien Lu, Kuan Hung Cho, Jian Kuen Wu, Chien Jang Wu, Yeun Chung Chang, Pan Chyr Yang

Research output: Contribution to journalArticle

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Abstract

Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation.

Original languageEnglish
Article numbere62762
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 2013 May 2

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Lewis Lung Carcinoma
Magnetic resonance
magnetic resonance imaging
carcinoma
Tumors
irradiation
lungs
Magnetic Resonance Imaging
Irradiation
Imaging techniques
neoplasms
Neoplasms
Radiotherapy
radiotherapy
diffusivity
Growth
Diffusion Magnetic Resonance Imaging
Microvessels
limbs (animal)
Inbred C57BL Mouse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI. / Cheng, Jason Chia Hsien; Yuan, Ang; Chen, Jyh Horng; Lu, Yi Chien; Cho, Kuan Hung; Wu, Jian Kuen; Wu, Chien Jang; Chang, Yeun Chung; Yang, Pan Chyr.

In: PLoS ONE, Vol. 8, No. 5, e62762, 02.05.2013.

Research output: Contribution to journalArticle

Cheng, Jason Chia Hsien ; Yuan, Ang ; Chen, Jyh Horng ; Lu, Yi Chien ; Cho, Kuan Hung ; Wu, Jian Kuen ; Wu, Chien Jang ; Chang, Yeun Chung ; Yang, Pan Chyr. / Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI. In: PLoS ONE. 2013 ; Vol. 8, No. 5.
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abstract = "Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8{\%} early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5{\%} increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2{\%} decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation.",
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AU - Cheng, Jason Chia Hsien

AU - Yuan, Ang

AU - Chen, Jyh Horng

AU - Lu, Yi Chien

AU - Cho, Kuan Hung

AU - Wu, Jian Kuen

AU - Wu, Chien Jang

AU - Chang, Yeun Chung

AU - Yang, Pan Chyr

PY - 2013/5/2

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N2 - Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation.

AB - Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation.

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