Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI

Jason Chia Hsien Cheng, Ang Yuan, Jyh Horng Chen, Yi Chien Lu, Kuan Hung Cho, Jian Kuen Wu, Chien Jang Wu, Yeun Chung Chang, Pan Chyr Yang

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Abstract

Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation.

Original languageEnglish
Article numbere62762
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 2013 May 2

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cheng, J. C. H., Yuan, A., Chen, J. H., Lu, Y. C., Cho, K. H., Wu, J. K., Wu, C. J., Chang, Y. C., & Yang, P. C. (2013). Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI. PLoS ONE, 8(5), [e62762]. https://doi.org/10.1371/journal.pone.0062762