Early activation of bradykinin B2 receptor aggravates reactive oxygen species generation and renal damage in ischemia/reperfusion injury

Wen Chih Chiang, Chiang-Ting Chien, Wan Wan Lin, Shuei Liong Lin, Yung Ming Chen, Chun Fu Lai, Kwan Dun Wu, Julie Chao, Tun Jun Tsai

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The kallikrein/kinin system is beneficial in ischemia/reperfusion injury in heart, controversial in brain, but detrimental in lung, liver, and intestine. We examined the role of the kallikrein/kinin system in acute ischemia/reperfusion renal injury induced by 40 min occlusion of the renal artery followed by reperfusion. Rats were infused with tissue kallikrein protein 5 days before (pretreated group) or after (treated group) ischemia. Two days later, the pretreated group exhibited the worst renal dysfunction, followed by the treated group, then the control group. Kallikrein increased tubular necrosis and inflammatory cell infiltration with generation of more tumor necrosis factor-α and monocyte chemoattractant protein-1. Reactive oxygen species (ROS), malondialdehyde, and reduced/oxidized glutathione measurement revealed that the oxidative stress was augmented by kallikrein administration in both ischemic and reperfusion phases. The groups with more ROS generation also had more apoptotic renal cells. The deleterious effects of kallikrein on ischemia/reperfusion injury were reversed by cotreatment with bradykinin B2 receptor (B2R) antagonist, but not B1 receptor antagonist, and were not associated with hemodynamic changes. We conclude that early activation of B2R augmented ROS generation in ischemia/reperfusion renal injury, resulting in subsequent apoptosis, inflammation, and tissue damage. This finding suggests the potential application of B2R antagonists in acute ischemic renal disease associated with bradykinin activation.

Original languageEnglish
Pages (from-to)1304-1314
Number of pages11
JournalFree Radical Biology and Medicine
Volume41
Issue number8
DOIs
Publication statusPublished - 2006 Oct 15

Fingerprint

Bradykinin B2 Receptors
Kallikreins
Reperfusion Injury
Reactive Oxygen Species
Chemical activation
Kidney
Kallikrein-Kinin System
Kinins
Reperfusion
Tissue Kallikreins
Oxidative stress
Glutathione Disulfide
Chemokine CCL2
Hemodynamics
Bradykinin
Renal Artery
Malondialdehyde
Infiltration
Liver
Intestines

Keywords

  • Apoptosis
  • Bradykinin
  • Free radicals
  • Inflammation
  • Ischemia/reperfusion
  • Kallikrein
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Early activation of bradykinin B2 receptor aggravates reactive oxygen species generation and renal damage in ischemia/reperfusion injury. / Chiang, Wen Chih; Chien, Chiang-Ting; Lin, Wan Wan; Lin, Shuei Liong; Chen, Yung Ming; Lai, Chun Fu; Wu, Kwan Dun; Chao, Julie; Tsai, Tun Jun.

In: Free Radical Biology and Medicine, Vol. 41, No. 8, 15.10.2006, p. 1304-1314.

Research output: Contribution to journalArticle

Chiang, Wen Chih ; Chien, Chiang-Ting ; Lin, Wan Wan ; Lin, Shuei Liong ; Chen, Yung Ming ; Lai, Chun Fu ; Wu, Kwan Dun ; Chao, Julie ; Tsai, Tun Jun. / Early activation of bradykinin B2 receptor aggravates reactive oxygen species generation and renal damage in ischemia/reperfusion injury. In: Free Radical Biology and Medicine. 2006 ; Vol. 41, No. 8. pp. 1304-1314.
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