Differential proteomics of monosodium urate crystals-induced inflammatory response in dissected murine air pouch membranes by iTRAQ technology

Chih Wei Chiu, Han Min Chen, Tzong Ta Wu, Ying Chu Shih, Kuo Kuei Huang, Ying Fei Tsai, Yi Ling Hsu, Sung Fang Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The precipitation of monosodium urate crystals within joints triggers an acute inflammatory reaction that is the root cause of gout. The inflammation induced by the injection of MSU crystals into the murine air pouch for 1, 3, and 5 h was examined by iTRAQ-based proteomic profiling. The iTRAQ-labeled peptides were fractionated by SCX, basic-RP or solution-IEF, followed by LC-MS/MS analysis. A total of 951 proteins were quantified from the total combined fractions. Among them, 317 proteins exhibited a differential expression, compared to that of the controls at one time point or more. The majority of the differentially expressed proteins were found in the sample after a 5-h MSU treatment. Western blot revealed that the expression levels of cathelin-related antimicrobial peptide and S100A9 were positively correlated with the time-course treated with MSU. Further analysis of GeneGO pathway demonstrated that these differentially expressed proteins are primarily related to the immune-related complement system and the tricarboxylic acid cycle. Moreover, seven genes from the TCA cycle were found to be significantly downregulated at the transcriptional level and its correlation with gout and possible therapeutic applications are worth further investigation. Last, we found that pyruvate carboxylation could be potential targets for antigout treatment.

Original languageEnglish
Pages (from-to)3338-3348
Number of pages11
JournalProteomics
Volume15
Issue number19
DOIs
Publication statusPublished - 2015 Oct 1

Keywords

  • Animal proteomics
  • Gout
  • Inflammation
  • Monosodium urate
  • Murine air pouch

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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