Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer

Paul M. Schneider, Kang Fang, Jack A. Roth, Mien Chie Hung, Susanna M. Chiocca, Kang Fang, John Manning

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

We studied non-small cell lung cancers (NSCLC) from 60 patients for abnormalities in the c-erbB-2 gene. Eleven human lung cancer cell lines, including four derived from small cell lung cancer (SCLC) and seven derived from NSCLC were also examined for altered c-erbB-2 gene expression. Southern blot analysis of paired tumor and normal lung samples demonstrated that amplification of the c-erbB-2 gene is rare in NSCLC (2/60) and not restricted to adenocarcinomas. One patient showed an EcoRI restriction fragment length polymorphism for the cerbB-2 locus. Four of four SCLC cell lines demonstrated minimal or nondetectable expression of c-erbB-2 mRNA compared to high levels of expression by seven of seven NSCLC lines. The highest expression levels were seen in four of four adenocarcinomas. We conclude that c-erbB-2 expression is different in SCLC compared to NSCLC and high expression ofc-erbB-2 is consistently present in lung adenocarcinomas.

Original languageEnglish
Pages (from-to)4968-4971
Number of pages4
JournalCancer Research
Volume49
Issue number18
Publication statusPublished - 1989 Sep 15

Fingerprint

erbB-2 Genes
Non-Small Cell Lung Carcinoma
Small Cell Lung Carcinoma
Adenocarcinoma
Cell Line
Southern Blotting
Restriction Fragment Length Polymorphisms
Lung Neoplasms
Gene Expression
Lung
Messenger RNA
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Schneider, P. M., Fang, K., Roth, J. A., Hung, M. C., Chiocca, S. M., Fang, K., & Manning, J. (1989). Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer. Cancer Research, 49(18), 4968-4971.

Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer. / Schneider, Paul M.; Fang, Kang; Roth, Jack A.; Hung, Mien Chie; Chiocca, Susanna M.; Fang, Kang; Manning, John.

In: Cancer Research, Vol. 49, No. 18, 15.09.1989, p. 4968-4971.

Research output: Contribution to journalArticle

Schneider, PM, Fang, K, Roth, JA, Hung, MC, Chiocca, SM, Fang, K & Manning, J 1989, 'Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer', Cancer Research, vol. 49, no. 18, pp. 4968-4971.
Schneider PM, Fang K, Roth JA, Hung MC, Chiocca SM, Fang K et al. Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer. Cancer Research. 1989 Sep 15;49(18):4968-4971.
Schneider, Paul M. ; Fang, Kang ; Roth, Jack A. ; Hung, Mien Chie ; Chiocca, Susanna M. ; Fang, Kang ; Manning, John. / Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer. In: Cancer Research. 1989 ; Vol. 49, No. 18. pp. 4968-4971.
@article{6bc1313e3fcd45ab97aaff510453e717,
title = "Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer",
abstract = "We studied non-small cell lung cancers (NSCLC) from 60 patients for abnormalities in the c-erbB-2 gene. Eleven human lung cancer cell lines, including four derived from small cell lung cancer (SCLC) and seven derived from NSCLC were also examined for altered c-erbB-2 gene expression. Southern blot analysis of paired tumor and normal lung samples demonstrated that amplification of the c-erbB-2 gene is rare in NSCLC (2/60) and not restricted to adenocarcinomas. One patient showed an EcoRI restriction fragment length polymorphism for the cerbB-2 locus. Four of four SCLC cell lines demonstrated minimal or nondetectable expression of c-erbB-2 mRNA compared to high levels of expression by seven of seven NSCLC lines. The highest expression levels were seen in four of four adenocarcinomas. We conclude that c-erbB-2 expression is different in SCLC compared to NSCLC and high expression ofc-erbB-2 is consistently present in lung adenocarcinomas.",
author = "Schneider, {Paul M.} and Kang Fang and Roth, {Jack A.} and Hung, {Mien Chie} and Chiocca, {Susanna M.} and Kang Fang and John Manning",
year = "1989",
month = "9",
day = "15",
language = "English",
volume = "49",
pages = "4968--4971",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "18",

}

TY - JOUR

T1 - Differential Expression of the c-erbB-2 Gene in Human Small Cell and Non-Small Cell Lung Cancer

AU - Schneider, Paul M.

AU - Fang, Kang

AU - Roth, Jack A.

AU - Hung, Mien Chie

AU - Chiocca, Susanna M.

AU - Fang, Kang

AU - Manning, John

PY - 1989/9/15

Y1 - 1989/9/15

N2 - We studied non-small cell lung cancers (NSCLC) from 60 patients for abnormalities in the c-erbB-2 gene. Eleven human lung cancer cell lines, including four derived from small cell lung cancer (SCLC) and seven derived from NSCLC were also examined for altered c-erbB-2 gene expression. Southern blot analysis of paired tumor and normal lung samples demonstrated that amplification of the c-erbB-2 gene is rare in NSCLC (2/60) and not restricted to adenocarcinomas. One patient showed an EcoRI restriction fragment length polymorphism for the cerbB-2 locus. Four of four SCLC cell lines demonstrated minimal or nondetectable expression of c-erbB-2 mRNA compared to high levels of expression by seven of seven NSCLC lines. The highest expression levels were seen in four of four adenocarcinomas. We conclude that c-erbB-2 expression is different in SCLC compared to NSCLC and high expression ofc-erbB-2 is consistently present in lung adenocarcinomas.

AB - We studied non-small cell lung cancers (NSCLC) from 60 patients for abnormalities in the c-erbB-2 gene. Eleven human lung cancer cell lines, including four derived from small cell lung cancer (SCLC) and seven derived from NSCLC were also examined for altered c-erbB-2 gene expression. Southern blot analysis of paired tumor and normal lung samples demonstrated that amplification of the c-erbB-2 gene is rare in NSCLC (2/60) and not restricted to adenocarcinomas. One patient showed an EcoRI restriction fragment length polymorphism for the cerbB-2 locus. Four of four SCLC cell lines demonstrated minimal or nondetectable expression of c-erbB-2 mRNA compared to high levels of expression by seven of seven NSCLC lines. The highest expression levels were seen in four of four adenocarcinomas. We conclude that c-erbB-2 expression is different in SCLC compared to NSCLC and high expression ofc-erbB-2 is consistently present in lung adenocarcinomas.

UR - http://www.scopus.com/inward/record.url?scp=0024421662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024421662&partnerID=8YFLogxK

M3 - Article

VL - 49

SP - 4968

EP - 4971

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 18

ER -