Differential effects of silver nanoparticles on two types of mitochondrion-rich ionocytes in zebrafish embryos

Jiun Lin Horng, Chih Ying Lee, Sian Tai Liu, Giun Yi Hung, Li Yih Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Silver nanoparticles (AgNPs) are increasingly used in our daily life and have become a potential environmental hazard. However, the toxic effects of AgNPs on the early stages of fish are not fully understood, and little is known about their effects on specific types of ionocytes. Using zebrafish embryos as a model, this study examined the effects (changes in cell number, morphology, NH4+ secretion and gene expression) of sublethal concentrations of AgNPs (0.1, 1, and 3 mg/L) on two major types of ionocytes: H+ pump-rich (HR) ionocytes, and Na+ pump-rich (NaR) ionocytes in the skin of embryos. After exposure to AgNPs for 96 h, the number of HR ionocytes significantly declined by 30% and 41% in the 1 and 3 mg/L AgNP groups, respectively. In addition, the apical opening of HR ionocytes became smaller, suggesting that AgNPs impaired the critical structure for ion transport. NH4+ secretion by HR ionocytes of embryos also declined significantly after AgNP exposure. In contrast, the number of NaR ionocytes increased by 29% and 43% in the 1 and 3 mg/L AgNP groups, respectively, while these cells deformed their shape. AgNPs altered mRNA levels of several ion channel and transporter genes involved in the functions of HR ionocytes and NaR ionocytes, and influenced hormone genes involved in regulating calcium homeostasis. This study shows that AgNPs can cause differential adverse effects on two types of ionocytes and the effects can threaten fish survival.

Original languageEnglish
Article number109244
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume252
DOIs
Publication statusPublished - 2022 Feb

Keywords

  • Embryo
  • Hormone
  • Ion regulation
  • Ionocyte
  • Mitochondria-rich cell
  • Silver nanoparticles

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Differential effects of silver nanoparticles on two types of mitochondrion-rich ionocytes in zebrafish embryos'. Together they form a unique fingerprint.

Cite this