Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics

Chieh Lin, Qian Xian Zhang, Yi Chun Yeh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Most disorders associated with tyrosine metabolism lead to elevated tyrosine levels in biological fluids. In this work, we used genetic engineering techniques to develop a simple and low-cost biosensor for tyrosine quantification. Here, two tyrosine-responsive promoters are used to differentially control the production of red and green fluorescent proteins signals in response to tyrosine levels in a culture. We also conducted a systematic examination of various modular components (promoters and ribosome binding sites) in order to ensure that our biosensor was capable of rapidly producing a pronounced fluorescence signal. To make the device more user-friendly, we developed a reagentless platform in which biocompatible agarose is used as an entrapment agent with cell sensors and growth media mounted within the gel matrix.

Original languageEnglish
Pages (from-to)1400-1404
Number of pages5
JournalAnalytical Methods
Volume11
Issue number10
DOIs
Publication statusPublished - 2019 Mar 14

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Biosensors
Tyrosine
Genetic engineering
Binding sites
Metabolism
Gels
Fluorescence
Proteins
Fluids
Sensors
Costs
Green Fluorescent Proteins
Sepharose
Binding Sites

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Cite this

Development of a whole-cell biosensor for the determination of tyrosine in urine for point-of-care diagnostics. / Lin, Chieh; Zhang, Qian Xian; Yeh, Yi Chun.

In: Analytical Methods, Vol. 11, No. 10, 14.03.2019, p. 1400-1404.

Research output: Contribution to journalArticle

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