Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ

Sin Ying Shen; Wei Chun Chang; Hsiang Heng Yi; Shinn Shong Tsai; Hung Jen Liu; Pei Chun Liao; Kuo Pin Chuang

doi:10.1016/j.vetimm.2015.08.002

Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ

Sin Ying Shen, Wei Chun Chang, Hsiang Heng Yi, Shinn Shong Tsai, Hung Jen Liu, Pei Chun Liao, Kuo Pin Chuang^*

^*Corresponding author for this work

Department of Life Science

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Chicken anemia virus (CAV) is a severe threat to the chicken industry and causes heavy economic losses worldwide. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant VP1 (rVP1) and pigeon interferon-γ (rPiIFN-γ). Results indicated that rPiIFN-γ enhanced humoral immunity elicited by rVP1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. When compared to chickens immunized with rVP1, inactivated vaccine, chickens immunized with rVP1. +. rPiIFN-γ showed faster and higher levels (. p<. 0.05) of antibody titer. The CAV challenge result showed that the rVP1. +. rPiIFN-γ vaccine prevent the reducing of hematocrit values in comparison with the rVP1 or inactivated groups. The relative fold inductions of mRNA expression of Th1-type (IFN-γ), but not Th2-type (IL-4) cytokines in splenocytes isolated from chickens immunized with rVP1. +. rPiIFN-γ were significantly higher than those of the rVP1 or inactivated vaccine groups. In conclusion, our study found that rPiIFN-γ can enhance both humoral and cellular immunity elicited by an rVP1 vaccine. The rVP1. +. rPiIFN-γ vaccine may provide a new strategy vaccine against CAV in chicken.

Original language	English
Pages (from-to)	200-204
Number of pages	5
Journal	Veterinary Immunology and Immunopathology
Volume	167
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2015.08.002
Publication status	Published - 2015 Oct 15

Keywords

CAV
Pigeon IFN-γ
Subunit vaccine

ASJC Scopus subject areas

Immunology
General Veterinary

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetimm.2015.08.002

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title = "Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ",

abstract = "Chicken anemia virus (CAV) is a severe threat to the chicken industry and causes heavy economic losses worldwide. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant VP1 (rVP1) and pigeon interferon-γ (rPiIFN-γ). Results indicated that rPiIFN-γ enhanced humoral immunity elicited by rVP1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. When compared to chickens immunized with rVP1, inactivated vaccine, chickens immunized with rVP1. +. rPiIFN-γ showed faster and higher levels (. p<. 0.05) of antibody titer. The CAV challenge result showed that the rVP1. +. rPiIFN-γ vaccine prevent the reducing of hematocrit values in comparison with the rVP1 or inactivated groups. The relative fold inductions of mRNA expression of Th1-type (IFN-γ), but not Th2-type (IL-4) cytokines in splenocytes isolated from chickens immunized with rVP1. +. rPiIFN-γ were significantly higher than those of the rVP1 or inactivated vaccine groups. In conclusion, our study found that rPiIFN-γ can enhance both humoral and cellular immunity elicited by an rVP1 vaccine. The rVP1. +. rPiIFN-γ vaccine may provide a new strategy vaccine against CAV in chicken.",

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author = "Shen, {Sin Ying} and Chang, {Wei Chun} and Yi, {Hsiang Heng} and Tsai, {Shinn Shong} and Liu, {Hung Jen} and Liao, {Pei Chun} and Chuang, {Kuo Pin}",

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AU - Shen, Sin Ying

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AU - Liu, Hung Jen

AU - Liao, Pei Chun

AU - Chuang, Kuo Pin

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AB - Chicken anemia virus (CAV) is a severe threat to the chicken industry and causes heavy economic losses worldwide. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant VP1 (rVP1) and pigeon interferon-γ (rPiIFN-γ). Results indicated that rPiIFN-γ enhanced humoral immunity elicited by rVP1 as early as 10 day after primary immunization and reach the high titer after secondary immunization. When compared to chickens immunized with rVP1, inactivated vaccine, chickens immunized with rVP1. +. rPiIFN-γ showed faster and higher levels (. p<. 0.05) of antibody titer. The CAV challenge result showed that the rVP1. +. rPiIFN-γ vaccine prevent the reducing of hematocrit values in comparison with the rVP1 or inactivated groups. The relative fold inductions of mRNA expression of Th1-type (IFN-γ), but not Th2-type (IL-4) cytokines in splenocytes isolated from chickens immunized with rVP1. +. rPiIFN-γ were significantly higher than those of the rVP1 or inactivated vaccine groups. In conclusion, our study found that rPiIFN-γ can enhance both humoral and cellular immunity elicited by an rVP1 vaccine. The rVP1. +. rPiIFN-γ vaccine may provide a new strategy vaccine against CAV in chicken.

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Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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