Development of a pigment-based whole-cell biosensor for the analysis of environmental copper

Pei Hsuan Chen, Chieh Lin, Kai Hong Guo, Yi-Chun Yeh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Using engineered microorganisms to detect heavy metals in the environment has proven to be highly effective and robust. This paper reports on the development of a novel microbial sensor for the detection of copper ions. To develop this microbial sensor, we screened and characterized various biological parts, including promoters, output signals, and hosts. In addition, we used the plant pigment betaxanthin to output fluorescent signals in order to reduce the detection time. The resulting whole-cell biosensor presented a good sensitivity when detecting copper ions in environmental samples including freshwater pond and tap water.

Original languageEnglish
Pages (from-to)29302-29305
Number of pages4
JournalRSC Advances
Volume7
Issue number47
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Biosensors
Pigments
Copper
Ions
Sensors
Ponds
Heavy Metals
Microorganisms
Heavy metals
Water
betaxanthin

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Development of a pigment-based whole-cell biosensor for the analysis of environmental copper. / Chen, Pei Hsuan; Lin, Chieh; Guo, Kai Hong; Yeh, Yi-Chun.

In: RSC Advances, Vol. 7, No. 47, 01.01.2017, p. 29302-29305.

Research output: Contribution to journalArticle

Chen, Pei Hsuan ; Lin, Chieh ; Guo, Kai Hong ; Yeh, Yi-Chun. / Development of a pigment-based whole-cell biosensor for the analysis of environmental copper. In: RSC Advances. 2017 ; Vol. 7, No. 47. pp. 29302-29305.
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