Development of a capillary electrophoresis-77 K luminescence detection system for online spectral identification

C. H. Lin, Y. L. Chung, Y. H. Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have demonstrated that capillary electrophoresis (CE) can be easily interfaced with 77 K luminescence spectroscopy (LS) for separation and online spectral identification of structurally similar analytes. This novel CE-LS apparatus consists of a regular CE system, instrumentation for LS and a specially designed capillary Dewar. When the separating molecules traverse into the cryostat detection window, liquid nitrogen is added, freezing the separating analyte zones within the capillary. At low temperature, detection limits are improved via signal averaging and the inherent increase in quantum yield at 77 K. We present the first application of the CE-LS system to structural isomers (2,3- and 3,4-methylenedioxymethamphetamine) and stereoisomers (trans- and cis-resveratrol). With this approach, the CE-LS interfacing provides a sensitive, accurate, rapid, simple and economic methodology for analytical chemistry.

Original languageEnglish
Pages (from-to)302-305
Number of pages4
JournalAnalyst
Volume126
Issue number3
DOIs
Publication statusPublished - 2001 Apr 5

Fingerprint

Online Systems
Capillary electrophoresis
Capillary Electrophoresis
luminescence
Luminescence
Spectrum Analysis
electrokinesis
spectroscopy
Spectroscopy
Resveratrol
Dewars
N-Methyl-3,4-methylenedioxyamphetamine
Stereoisomerism
Cryostats
Quantum yield
Liquid nitrogen
Freezing
Isomers
instrumentation
freezing

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Development of a capillary electrophoresis-77 K luminescence detection system for online spectral identification. / Lin, C. H.; Chung, Y. L.; Chen, Y. H.

In: Analyst, Vol. 126, No. 3, 05.04.2001, p. 302-305.

Research output: Contribution to journalArticle

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