Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae)

Shohei Komaki, Takeshi Igawa, Masafumi Nozawa, Si Min Lin, Shohei Oumi, Masayuki Sumida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalGenes and Genetic Systems
Volume89
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Amphibians
Anura
Microsatellite Repeats
Genetic Markers
Islands
Population
Demography
Phylogeography
Reptiles
Alleles
Ions

Keywords

  • Amphibian
  • Genetic diversity
  • Island species
  • Microsatellite markers
  • Population genetics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae). / Komaki, Shohei; Igawa, Takeshi; Nozawa, Masafumi; Lin, Si Min; Oumi, Shohei; Sumida, Masayuki.

In: Genes and Genetic Systems, Vol. 89, No. 1, 01.01.2014, p. 35-39.

Research output: Contribution to journalArticle

Komaki, Shohei ; Igawa, Takeshi ; Nozawa, Masafumi ; Lin, Si Min ; Oumi, Shohei ; Sumida, Masayuki. / Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae). In: Genes and Genetic Systems. 2014 ; Vol. 89, No. 1. pp. 35-39.
@article{83839506a7f740beae4392182108f110,
title = "Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae)",
abstract = "Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.",
keywords = "Amphibian, Genetic diversity, Island species, Microsatellite markers, Population genetics",
author = "Shohei Komaki and Takeshi Igawa and Masafumi Nozawa and Lin, {Si Min} and Shohei Oumi and Masayuki Sumida",
year = "2014",
month = "1",
day = "1",
doi = "10.1266/ggs.89.35",
language = "English",
volume = "89",
pages = "35--39",
journal = "Genes and Genetic Systems",
issn = "1341-7568",
publisher = "Genetics Society of Japan",
number = "1",

}

TY - JOUR

T1 - Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae)

AU - Komaki, Shohei

AU - Igawa, Takeshi

AU - Nozawa, Masafumi

AU - Lin, Si Min

AU - Oumi, Shohei

AU - Sumida, Masayuki

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.

AB - Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.

KW - Amphibian

KW - Genetic diversity

KW - Island species

KW - Microsatellite markers

KW - Population genetics

UR - http://www.scopus.com/inward/record.url?scp=84900409813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900409813&partnerID=8YFLogxK

U2 - 10.1266/ggs.89.35

DO - 10.1266/ggs.89.35

M3 - Article

C2 - 24817760

AN - SCOPUS:84900409813

VL - 89

SP - 35

EP - 39

JO - Genes and Genetic Systems

JF - Genes and Genetic Systems

SN - 1341-7568

IS - 1

ER -