Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

An Kai Su; Cheng Huang Lin

doi:10.1016/S1570-0232(02)00849-8

Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

An Kai Su
, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 μg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B₂ tablet.

Original language	English
Pages (from-to)	39-46
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	785
Issue number	1
DOIs	https://doi.org/10.1016/S1570-0232(02)00849-8
Publication status	Published - 2003 Feb 25

Keywords

Blue light emitting diode
Riboflavin

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/S1570-0232(02)00849-8

Cite this

Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique. / Su, An Kai; Lin, Cheng Huang.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 785, No. 1, 25.02.2003, p. 39-46.

Research output: Contribution to journal › Article › peer-review

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abstract = "A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 μg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B2 tablet.",

keywords = "Blue light emitting diode, Riboflavin",

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note = "Funding Information: This work was supported by a grant from the National Science Council of Taiwan under contract no. NSC-90-2113-M-003-020.",

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AU - Lin, Cheng Huang

N1 - Funding Information: This work was supported by a grant from the National Science Council of Taiwan under contract no. NSC-90-2113-M-003-020.

PY - 2003/2/25

Y1 - 2003/2/25

N2 - A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 μg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B2 tablet.

AB - A simple, inexpensive and reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis-light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 μg/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period of 9 h after the ingestion of a vitamin B2 tablet.

KW - Blue light emitting diode

KW - Riboflavin

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Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

Abstract

Keywords

ASJC Scopus subject areas

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