Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

Ching Fang; Ju Tsung Liu; Shiu Huey Chou; Cheng Huang Lin

doi:10.1002/elps.200390119

Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

Ching Fang, Ju Tsung Liu, Shiu Huey Chou, Cheng Huang Lin^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD₅₀). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.

Original language	English
Pages (from-to)	1031-1037
Number of pages	7
Journal	Electrophoresis
Volume	24
Issue number	6
DOIs	https://doi.org/10.1002/elps.200390119
Publication status	Published - 2003 Mar

Keywords

Cation-selective exhaustive injection
Lysergic acid diethylamide (LSD)
Mouse blood sample
Sweeping micellar electrokinetic chromatography

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

Access to Document

10.1002/elps.200390119

Cite this

@article{9208c3f0c1ff4ca1882699822dc13eee,

title = "Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography",

abstract = "The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD50). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.",

keywords = "Cation-selective exhaustive injection, Lysergic acid diethylamide (LSD), Mouse blood sample, Sweeping micellar electrokinetic chromatography",

author = "Ching Fang and Liu, {Ju Tsung} and Chou, {Shiu Huey} and Lin, {Cheng Huang}",

year = "2003",

month = mar,

doi = "10.1002/elps.200390119",

language = "English",

volume = "24",

pages = "1031--1037",

journal = "Electrophoresis",

issn = "0173-0835",

publisher = "Wiley-VCH Verlag",

number = "6",

}

TY - JOUR

T1 - Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

AU - Fang, Ching

AU - Liu, Ju Tsung

AU - Chou, Shiu Huey

AU - Lin, Cheng Huang

PY - 2003/3

Y1 - 2003/3

N2 - The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD50). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.

AB - The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD50). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.

KW - Cation-selective exhaustive injection

KW - Lysergic acid diethylamide (LSD)

KW - Mouse blood sample

KW - Sweeping micellar electrokinetic chromatography

UR - http://www.scopus.com/inward/record.url?scp=0347765631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347765631&partnerID=8YFLogxK

U2 - 10.1002/elps.200390119

DO - 10.1002/elps.200390119

M3 - Article

C2 - 12658692

AN - SCOPUS:0347765631

SN - 0173-0835

VL - 24

SP - 1031

EP - 1037

JO - Electrophoresis

JF - Electrophoresis

IS - 6

ER -

Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this