Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography

Ching Fang, Ju Tsung Liu, Shiu Huey Chou, Cheng Huang Lin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD50). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.

Original languageEnglish
Pages (from-to)1031-1037
Number of pages7
JournalElectrophoresis
Volume24
Issue number6
DOIs
Publication statusPublished - 2003 Mar 1

Keywords

  • Cation-selective exhaustive injection
  • Lysergic acid diethylamide (LSD)
  • Mouse blood sample
  • Sweeping micellar electrokinetic chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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