TY - JOUR
T1 - Determination of lysergic acid diethylamide (LSD) by application of online 77 K fluorescence spectroscopy and a sweeping technique in micellar electrokinetic chromatography
AU - Fang, Ching
AU - Liu, Ju Tsung
AU - Lin, Cheng Huang
N1 - Funding Information:
This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-89-2113-M-003-016. Permission was obtained from Pharmaceutical Affairs, Department of Health, Taiwan (License Number: ARR089000035).
PY - 2002/10/16
Y1 - 2002/10/16
N2 - The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.
AB - The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.
KW - Capillary electrophoresis
KW - Iso-lysergic acid diethylamide (iso-LSD)
KW - Low temperature fluorescence spectroscopy
KW - Lysergic acid N,N-methylpropylamide (LAMPA)
KW - Lysergic acid diethylamide (LSD)
KW - Sweeping
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U2 - 10.1016/S0039-9140(02)00380-6
DO - 10.1016/S0039-9140(02)00380-6
M3 - Article
AN - SCOPUS:0037120743
SN - 0039-9140
VL - 58
SP - 691
EP - 699
JO - Talanta
JF - Talanta
IS - 4
ER -