Determination of lysergic acid diethylamide (LSD) by application of online 77 K fluorescence spectroscopy and a sweeping technique in micellar electrokinetic chromatography

Ching Fang, Ju Tsung Liu, Cheng-Huang Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.

Original languageEnglish
Pages (from-to)691-699
Number of pages9
JournalTalanta
Volume58
Issue number4
DOIs
Publication statusPublished - 2002 Oct 16

Fingerprint

Capillary electrophoresis
Lysergic Acid Diethylamide
Fluorescence Spectrometry
Fluorescence spectroscopy
Chromatography
Low temperature effects
Capillary Electrophoresis
Temperature
Cations
Kinetics
Limit of Detection
Urine
Injections

Keywords

  • Capillary electrophoresis
  • Iso-lysergic acid diethylamide (iso-LSD)
  • Low temperature fluorescence spectroscopy
  • Lysergic acid N,N-methylpropylamide (LAMPA)
  • Lysergic acid diethylamide (LSD)
  • Sweeping

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Determination of lysergic acid diethylamide (LSD) by application of online 77 K fluorescence spectroscopy and a sweeping technique in micellar electrokinetic chromatography",
abstract = "The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.",
keywords = "Capillary electrophoresis, Iso-lysergic acid diethylamide (iso-LSD), Low temperature fluorescence spectroscopy, Lysergic acid N,N-methylpropylamide (LAMPA), Lysergic acid diethylamide (LSD), Sweeping",
author = "Ching Fang and Liu, {Ju Tsung} and Cheng-Huang Lin",
year = "2002",
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TY - JOUR

T1 - Determination of lysergic acid diethylamide (LSD) by application of online 77 K fluorescence spectroscopy and a sweeping technique in micellar electrokinetic chromatography

AU - Fang, Ching

AU - Liu, Ju Tsung

AU - Lin, Cheng-Huang

PY - 2002/10/16

Y1 - 2002/10/16

N2 - The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.

AB - The principal advantage of the use of Shopl'skii effect (low temperature spectrum) is that spectral sharpening occurs both in absorption and emission. However, thus far using the technique of capillary electrophoresis/low temperature fluorescence spectroscopy (CE/LTFS) either at 77 or 4.2 K remains difficult to obtain an on-line spectrum, if the analyte is present at low concentration. This paper examines the feasibility of combining the techniques of online concentration and CE/LTFS to identify LSD and related compounds in urine at 77 K. To improve sensitivity, sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electro-kinetic chromatography (CSEI-sweep-MEKC) were used for on-line concentration which resulted in detection limits of ∼ 20 ∼ 60 ppt, respectively.

KW - Capillary electrophoresis

KW - Iso-lysergic acid diethylamide (iso-LSD)

KW - Low temperature fluorescence spectroscopy

KW - Lysergic acid N,N-methylpropylamide (LAMPA)

KW - Lysergic acid diethylamide (LSD)

KW - Sweeping

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U2 - 10.1016/S0039-9140(02)00380-6

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JF - Talanta

SN - 0039-9140

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