Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo; Pei Hsuan Chen; Chieh Lin; Chien Fu Chen; I. Ren Lee; Yi Chun Yeh

doi:10.1021/acssensors.7b00931

Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Kai Hong Guo
, Pei Hsuan Chen
, Chieh Lin
, Chien Fu Chen
, I. Ren Lee
, Yi Chun Yeh^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

Original language	English
Pages (from-to)	744-748
Number of pages	5
Journal	ACS Sensors
Volume	3
Issue number	4
DOIs	https://doi.org/10.1021/acssensors.7b00931
Publication status	Published - 2018 Apr 27

Keywords

Cupriavidus metallidurans
cueR
paper device
smartphone-based diagnostic system
whole-cell biosensor

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/acssensors.7b00931

Cite this

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title = "Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device",

abstract = "This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.",

keywords = "Cupriavidus metallidurans, cueR, paper device, smartphone-based diagnostic system, whole-cell biosensor",

author = "Guo, \{Kai Hong\} and Chen, \{Pei Hsuan\} and Chieh Lin and Chen, \{Chien Fu\} and Lee, \{I. Ren\} and Yeh, \{Yi Chun\}",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = apr,

day = "27",

doi = "10.1021/acssensors.7b00931",

language = "English",

volume = "3",

pages = "744--748",

journal = "ACS Sensors",

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publisher = "American Chemical Society",

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T1 - Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

AU - Guo, Kai Hong

AU - Chen, Pei Hsuan

AU - Lin, Chieh

AU - Chen, Chien Fu

AU - Lee, I. Ren

AU - Yeh, Yi Chun

PY - 2018/4/27

Y1 - 2018/4/27

N2 - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

AB - This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine.

KW - Cupriavidus metallidurans

KW - cueR

KW - paper device

KW - smartphone-based diagnostic system

KW - whole-cell biosensor

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U2 - 10.1021/acssensors.7b00931

DO - 10.1021/acssensors.7b00931

M3 - Article

C2 - 29589435

AN - SCOPUS:85045875539

SN - 2379-3694

VL - 3

SP - 744

EP - 748

JO - ACS Sensors

JF - ACS Sensors

IS - 4

ER -

Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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